现代炼丹术一基因工程药物 B-gal B-gal Coning of human insulin in a bacterial host Insulin Insulin [after Griffiths et al 1996) A chain E chain AmpR Transform into E.coli HumanA-&B-chains are cloned separately. Each is attached to the plasmid B-gal: B-gal [+colonies carry cloned insulin genes. 基因工程胰岛素的生产原理 Culture cells Active insulin NH2 COOH NH2 -COOH Purify B-gal-insulin Disulfide bond fusion proteins When cysteine residues are oxidized, A-&B-chains spontaneously re-fold. Purify A and B chains A chain B chain 36现代炼丹术—基因工程药物 基因工程胰岛素的生产原理 36 生物化学与分子生物学教研室