The idea To examine the utilization and relative flux rates of each metabolic reaction in a wide range of simulated environmental conditions 30,000 randomly and uniformly chosen optimal growth conditions(randomly assigning values for metabolic-uptake reactions and all single-carbon-source minimal medium conditions sufficient for growth Using fba on in silico models H pylori ■E.cOl ■S. cerevisiaeThe idea ◼ To examine the utilization and relative flux rates of each metabolic reaction in a wide range of simulated environmental conditions ◼ 30,000 randomly and uniformly chosen optimal growth conditions (randomly assigning values for metabolic-uptake reactions) ◼ and all single-carbon-source minimal medium conditions sufficient for growth ◼ Using FBA on in silico models: ◼ H. pylori ◼ E. coli ◼ S. cerevisiae