23.2 Group I introns undertake self- splicing by transesterification Exon 1 Intron Exon 2 Figure 23. 1 Splicing of the Tetrahymena 35S rRNA precursor Gel electrophoresis Transcript can be followed by gel 35S RNA electrophoresis. The removal of the intron is revealed by the Splicing appearance of a rapidly moving small band. When the intron becomes circular. it Cyclization electrophoreses more slowly, as seen by a higher band Circular intron unear intron 消当Figure 23.1 Splicing of the Tetrahymena 35S rRNA precursor can be followed by gel electrophoresis. The removal of the intron is revealed by the appearance of a rapidly moving small band. When the intron becomes circular, it electrophoreses more slowly, as seen by a higher band. 23.2 Group I introns undertake self￾splicing by transesterification