Vol.28 No.1 郑裕东等:几种骨组织工程复合支架材料在模拟生理溶液中的生物矿化性能比较 ·67· production by human osteoblasts cultured on biodegradable and in vitro characterisation of novel bioresorbable and bioac- polymers applicable for tissue engineering.Blomaterlals, tive composite materials based on polylactide foams and Bio 2003,24:1213 glass(r for tissue engineering applications.Bomaterials, [3]Hutmacher D W.Scaffolds in tissue engineering bone and car- 2002,23:3871 tilage.Biomaterials,2000,21:2529 [11]Chua T M G,Ortone D G,Hollister S J.et al.Mechanical [4]Williams S F,Martin D P,Horowitz D M,et al.Peoples, and in vivo performance of hydroxyapatite implants with con- PHA applications:addressing the price performance issue I. trolled architectures.Blomaterials,2002,23:1283 Tissue engineering,Int J Biol Macromolec,1999,25:111 [12]郑裕东,王迎军,任耀彬,等,PHBV/sol-gel Bioglass骨组织 [5]Zhao K,Deng Y.Chen J C.et al.Polyhydroxyalkanoate 工程支架材料在模拟生理溶液中的化学反应研究.高等学 (PHA)scaffolds with good mechanical properties and biocom- 校化学学报,2003,24(7):1325 patibility.Biomaterials,2003,24:1041 [13]Yoshie N,Nakasato K,Fujiwara M.Effect of low molecular [6】郑裕东,钟膏华,马文石.用活性污泥生物合成聚羟基烷酸 weight additives on enzymatic degradation of poly 3-hydroxy- 酯的结构表征.高等学校化学学报,2000,21:991 butyrate.Polymer,2000,41:3227 [7]Zheng Y D,Chen X F,Wang YJ.The sol-gel derived bioac- [14]Kusaka S,Iwata T,Doi Y.Properties and biodegradability of tived glass/polyhydroxyalkanoates composites for bone tissue ultra-high-molecular-weight poly [R )-3-hydroxybutyrate engineering scaffolds//5 th Asian Symposium on Biomedical produced by a recombinant Escherichia col.Int J Biol Macro- Materials.Hong Kong,2001:337 molec,1999,25:87 [8]陈中华,聚羟基烷酸酯生物活性复合材料的改性与研究 [15]Regina M.Filgueiras T,LaTorre G.et al.Solution effects on [学位论文].广州:广州华南理工大学,2004 the surface reaction of three bioactive glass compostions.J [9]Ni J,Wang M.In vitro evaluation of hydroxyapatite rein Biomed Mater Res,1993,27:1485 forced polyhydroxybutyrate composite.Mater Sci Eng C. [16]Pereira MM,Clark A E.Hench LL.Calcium phosphate for- 2002,20:101 mation on sol-gel-derived bioactive glasses in vitro.J Biomed [10]Roether J A,Boccaccini A R.Hench LL,et al.Development Mater Res,1994,28:693 Biomineralization of three kinds of porous composites for bone tissue engineering scaffolds in simulated body fluid ZHENG Yudong,WANG Yingjun2),CHEN Zhonghua2),CHEN Xiaofen2,WU Gang?,XIE Jianzin) 1)Materials Science and Engineering School,University of Science and Technology Beijing,Beijing 100083,China 2)School of Materials Science and Engineering,South China University of Technology,Guangzhou 510640,China ABSTRACT Three kinds of porous composites based on poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV)and bioactive glass(BG),tricalcium phosphate (TCP),hydroxyapatite (HA)respectively were prepared for born tissue engineering scaffolds.The chemical reactions and in vitro degradation of PHBV/ BG,PHBV/TCP and PHBV/HA porous materials under physiological condition were assessed and com- pared.The changes in surface micromorphology,composition and structure of the composite scaffolds were investigated before and after immersed in simulated body fluid (SBF)solution.It was found that biodegra- dation occurred for three kinds of scaffolds which led to the weight loss of the scaffolds after immersed in SBF solution.The biomineralized reaction and ion exchange reaction occurred in PHBV/BG and PHBV/ TCP scaffolds in SBF solution,and the mineralized layer formed on the surface of those scaffolds which was analyzed as hydroxyl carbonate apatite (HCA).There was little biomineralized reaction appeared on PHBV/HA scaffolds during immersed in SBF sulotion. KEY WORDS biomineralization;polyhydroxyalkonate;bioactive glass;B-tricalcium phosphate;hydrox- yapatite;simulated body fluid心 郑裕东等 几种骨组织工程复合支架材料在模拟生理溶液中的生物矿化性能比较 《记 怡 以 , , 反战 吧 场 , , 认 , 田 运 , , , 凡气 主 昭 如 南 滋 飞 , 比 , , 〔 , 呢 , 公 山 称 盯 讲 让 目 , , 〔 郑裕东 钟青华 , 马文石 用活性污泥生物合成聚羚基烧酸 酿的结构表征 高称学校化学学报 , 呀 , , 一 找 记 哪 卯 肠 砚认 巧飞 斌 即 , 【 陈中华 聚 轻 基烷 酸醋生 物活性复合材 料 的 改性与研 究 【学位论文 广州 广州华南理工大学 , , 叨 叩 , , , 〕 二 , , 心 】 已 呀 昭 叩 以 目 , , 〕 , , , 玉 以 , , 〔 郑裕东 , 王迎军 , 任耀彬 , 等 旧 一 骨组 织 工程支架材料在模拟生理溶液 中的化学反应研究 高等学 校化学学报 , , 〔 , , 二 卯纷 一 衍 抑砚 , , , , 七 功 己 一 一 〔 一 卜 二 卿 成 盛 · , , 〕 班 , 瑙 , , 豁 翻 , , , 阮 , “ 这 卜 卜 巧 】 胎 拍 目 川 五 汉 , 长 蔚 。 , 月万 殊 。 。 ’ , 爪 。 , 口 俞鳍 , 正 艺 吃 】 , 昭 , 吨 , 二 吃 呢 , , , 卯 一 一 一 尹 , , , , , 吃 · 芍 尽 一