Biosynthesis of Pyridoxal Phosphate Decarboxylation of b-keto ester yields 1-amino-3- hydroxyacetone 3-phosphate 0 a-Ketoglutarate NAD+ Glutamate NADH/H+ C=0 H3N H H OH H OH CH2OPO32- CH2OPO32- 3-Hydroxy-4-phospho- 4-Phosphohydroxy- hydroxy-2-ketobutyrate threonine c02 H3N H A H3N 0P032 C=0 1-Amino-3-hydroxy- CH20P032 acetone 3-phosphate A B-keto ester▪ Decarboxylation of b-keto ester yields 1-amino-3- hydroxyacetone 3-phosphate Biosynthesis of Pyridoxal Phosphate