上浒文通大学 Mechanism of isoniazid Shanghai Jiao Tong University Prodrug Activated by bacterial catalase(过氧化氢酶)- peroxidase(过氧化物酶)enzyme called KatG KatG couples the isonicotinic acyl with NADH 胺腺嘌呤二核苷酸，辅酶) Form isonicotinic acyl-NADH complex Complex binds tightly to ketoenoylreductase 醇还原酶) Blocking the natural enoyl-AcpM substrate and the action of fatty acid synthase (acyl carrier protein) the process inhibits the synthesis of mycolic acid 枝菌酸)，required for the mycobaterial cell wall.. AcpM:acyl carrier protein (JBC,2003,278(23):20647-54)Shanghai Jiao Tong University Mechanism of isoniazid Prodrug Activated by bacterial catalase（过氧化氢酶）- peroxidase（过氧化物酶） enzyme called KatG KatG couples the isonicotinic acyl with NADH（烟酰 胺腺嘌呤二核苷酸，辅酶 I） Form isonicotinic acyl-NADH complex Complex binds tightly to ketoenoylreductase（酮烯 醇还原酶） Blocking the natural enoyl-AcpM substrate and the action of fatty acid synthase （acyl carrier protein) the process inhibits the synthesis of mycolic acid（分 枝菌酸）, required for the mycobaterial cell wall. AcpM: acyl carrier protein (JBC, 2003, 278(23):20647-54)