Chapter 8 Enzymes:Basic Concepts and Kinetics 12 Multiple substrate enzyme reactions are divided into two classes: A) sequential displacement and double displacement. B) double displacement and concerted displacement. c) sequential displacement and ping-pong. D) a and c. E) None of the above. Ans:D Section:8.4 13 What type(s)of inhibition can be reversed? A) competitive D) All of the above B) noncompetitive E) None of the above C) mixed Ans:D Section:8.5 14 In this type of inhibition,the inhibitor can only bind to the ES complex to form an ESI complex. A) competitive D) uncompetitive B) noncompetitive E) None of the above. C)mixed Ans:D Section:8.5 15 Riboflavin is a water-soluble organic substance that is not synthesized by humans. Metabolically,it is chemically converted into a substance called flavin adenine dinucleotide, which is required by succinate dehydrogenase.Which of the following statements is most correct? A Riboflavin is a coenzyme. B) Flavin adenine dinucleotide is a vitamin. C) Succiniate dehydrogenase is a coenzyme. D) Flavin adenine dinucleotide is a coenzyme. Ans:D Section:8.1 Short-Answer Questions 1 In many enzyme assays,the natural substrate and product are not used.Why? Ans:Many products are difficult to measure accurately.Some are simply difficult to measure, while others are difficult to discern against the background of other molecules present in the reaction.Instead,substrates are chosen that the enzyme can still process but that result in products that can be easily measured.For example,substrates are chosen that result in products that are colored and can be detected spectrophotometrically. Section:8.1 2 Give an example of a reaction catalyzed by a hydrolase. Ans:Hydrolases catalyze a reaction where a water molecule is added across some linkage that was originally formed by the removal of water.Some examples are the hydrolysis of an ester,the hydrolysis of a peptide bond,the hydrolysis of a glycoside bond,etc.A diagram similar to textbook Section 8.1 are examples. Section:AppendixChapter 8 Enzymes: Basic Concepts and Kinetics 5 12 Multiple substrate enzyme reactions are divided into two classes: A) sequential displacement and double displacement. B) double displacement and concerted displacement. C) sequential displacement and ping-pong. D) a and c. E) None of the above. Ans: D Section: 8.4 13 What type(s) of inhibition can be reversed? A) competitive D) All of the above B) noncompetitive E) None of the above C) mixed Ans: D Section: 8.5 14 In this type of inhibition, the inhibitor can only bind to the ES complex to form an ESI complex. A) competitive D) uncompetitive B) noncompetitive E) None of the above. C) mixed Ans: D Section: 8.5 15 Riboflavin is a water-soluble organic substance that is not synthesized by humans. Metabolically, it is chemically converted into a substance called flavin adenine dinucleotide, which is required by succinate dehydrogenase. Which of the following statements is most correct? A) Riboflavin is a coenzyme. B) Flavin adenine dinucleotide is a vitamin. C) Succiniate dehydrogenase is a coenzyme. D) Flavin adenine dinucleotide is a coenzyme. Ans: D Section: 8.1 Short-Answer Questions 1 In many enzyme assays, the natural substrate and product are not used. Why? Ans: Many products are difficult to measure accurately. Some are simply difficult to measure, while others are difficult to discern against the background of other molecules present in the reaction. Instead, substrates are chosen that the enzyme can still process but that result in products that can be easily measured. For example, substrates are chosen that result in products that are colored and can be detected spectrophotometrically. Section: 8.1 2 Give an example of a reaction catalyzed by a hydrolase. Ans: Hydrolases catalyze a reaction where a water molecule is added across some linkage that was originally formed by the removal of water. Some examples are the hydrolysis of an ester, the hydrolysis of a peptide bond, the hydrolysis of a glycoside bond, etc. A diagram similar to textbook Section 8.1 are examples. Section: Appendix