Heat Moist heat Exhaust valve (to remove Steam to Safety Pressure Operating valve(controls steam steam after sterilization) chamber valve gauge from jacket to chamber) denatures proteins Autoclave: Steam Steam under chambe pressure Perforated shelf Sediment screen Steam jacket Thermometer Automatic ejector valve is Pressure regulator thermostatically controlled for steam supply and closes on contact with pure steam when air is To waste line exhausted. Steam supply Figure 7.2 • Moist heat denatures proteins • Autoclave: Steam under pressure Heat Figure 7.2