Multiple cloning sites Multiple restriction sites enable the convenient insertion of target DNA into a vector Lac promoter Amp MCS(Multiple cloning sites 多克隆位点) pUC18 lacz (3kb) or Sall Smal Hindi EcorI SacI KpnI Xmal BamhI XbaI Accl PstI SphI ACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA ThrAsn ser s e r Val Pro Gly Asp Pro Leu Glu Ser Thr Cys Arg His Ala Ser Lac zMultiple cloning sites Multiple restriction sites enable the convenient insertion of target DNA into a vector Ampr ori pUC18 (3 kb) MCS (Multiple cloning sites, 多克隆位点） Lac promoter lacZ’ …ACGAATTCGAGCTCGGTACCCGGGGATCCTCTAGAGTCGACCTGCAGGCATGCA… . T h rA s n S er S e r Val Pro Gly Asp Pro Leu Glu Ser Thr Cys Arg His Ala Ser… EcoRI SacI KpnI SmaI XmaI BamHI XbaI SalI HincII AccI PstI SphI Lac Z