Foreign DNA to be inserted 分离目的基因（分） Joining Plasmid vector Antibiotic-resistance gene 体外重组（切、连、转） Recombinant DNA molecule Introduction into host cell 扩增繁殖（选、鉴） Selection for cells containing recombinant DNA molecules by growth in the presence of antibiotic t O FIGURE 5-11 The cloning of DNA in a plasmid. 分离目的基因（分） 体外重组（切、连、转） 扩增繁殖（选、鉴）