Teaching plan of Soochow University Subjects:Biochemistry Experiment Class:2019 Pharmaceutical sciences Date:2020.11. 13/Fri/5-8 Chapter 4 Text page:N/A Teaching duration:200 Title Determination of Cholesterol In HDL (1).Master the technique to separate HDL from other types of lipoproteins Teaching goal (2).Understand the method to extract and determine cholesterol concentration in HDL (3).Learn the clinical significance of HDL l、Content: (1).Review the biochemistry of lipoprotein 20' Teaching (2).The method to separate HDL from other type of lipoprotein content、 10 Teaching (3).The principle of cholesterol extraction and quantification method、 20 Teaching (4)Student's experiment 150 emphasis、 difficulty、 2、Key point: (1).Significance of Lipoprotein. time distribution (2).Cholesterol extraction method 3.Teaching method Teacher should explain and display the experiment according to powerpoint Teaching Powerpoint,glass pipet,centrifuge,spectrophotometer instrument 1. What kinds of lipoprotein are found in our blood stream? Question and quiz in 2.What is the source and chemical content of these lipoproteins? class How to adjust diet for patients with increased blood cholesterol level.Teaching plan of Soochow University Subjects：Biochemistry Experiment Class：2019 Pharmaceutical sciences Date：2020. 11. 13/Fri/5-8 Chapter 4 Text page：N/A Teaching duration: 200 Title Determination of Cholesterol In HDL Teaching goal (1). Master the technique to separate HDL from other types of lipoproteins (2). Understand the method to extract and determine cholesterol concentration in HDL (3). Learn the clinical significance of HDL Teaching content、 Teaching method、 Teaching emphasis、 difficulty、 time distribution 1、Content： (1). Review the biochemistry of lipoprotein 20’ (2). The method to separate HDL from other type of lipoprotein 10’ (3). The principle of cholesterol extraction and quantification 20’ (4) Student’s experiment 150’ 2、Key point： (1). Significance of Lipoprotein. (2). Cholesterol extraction method 3、Teaching method ： Teacher should explain and display the experiment according to powerpoint . Teaching instrument Powerpoint,glass pipet, centrifuge, spectrophotometer Question and quiz in class 1. What kinds of lipoprotein are found in our blood stream? 2. What is the source and chemical content of these lipoproteins? 3. How to adjust diet for patients with increased blood cholesterol level