引物设计原则 Sequence: Length of PCR product should ideally be less than 1 50 bp Avoid complementary sequences within and between nd rimers ar probes ■ Avoid mismatches I Avoid a 3 end t as this has a greater tolerance of mismatch Length ■18-30 nucleotides GC content 4060% ■T-2Cx(A++4℃x(C+G PRIMER3 PRIMERS PRIMER EXPRESS引物设计原则