s Specimen preparation for TEM Standard method: Fixation: Glutaraldehyde( proteins cross-linking),osmium tetroxide(lipid), Dehydration(in alcohol) and embedding within epoxy plastic called epon Section: 0.02-0.1 u m, cut by glass or diamond Section mounting on grids and stain by electron dyes, which provide the mass thickness required to scatter the electron beam: uranyl acetate and lead citrate or be treated with metal-tagged antibody ect. Or a number of cytochemical procedures to detect some ei enzymes as acid phosphatase* Specimen preparation for TEM Standard method: Fixation: Glutaraldehyde ( proteins cross-linking), osmium tetroxide (lipid), Dehydration (in alcohol) and embedding within epoxy plastic called Epon Section: 0.02-0.1μm, cut by glass or diamond Section mounting on grids and stain by electron dyes, which provide the mass thickness required to scatter the electron beam: uranyl acetate and lead citrate, or be treated with metal-tagged antibody ect. Or a number of cytochemical procedures to detect some enzymes as acid phosphatase