微生物与感染 Journalof microbes and Infections, December25,2015,10(6)365-370 验效果,本研究在实验课结束后针对教学对象 2012级)共36名同学进行问卷调查和统计分析,结 复旦大学临床医学专业六年制2个年级(2011级和果见表5。 表3AP10S与16 SrRNA鉴定及药敏试验结果 Tab 3 Identification and antimicrobial susceptibility testing results No. API lOS identification Antimicrobial susceptibility testing 16S rRNA identification STR GEN 1 Escherichia coli I Escherichia coli 3 Serratia odorifera or Escherichia coli l Escherichia coli 4 Escherichia coli l Escherichia coli 5 Escherichia coli I Escherichia coli R R 6 Escherichia coli l Cronobacter sakazakii 7 Serratia odorifera or Escherichia coli I Escherichia coli 8 9 Escherichia coli or Shigella spp Escherichia coli R 10 Escherichia coli 2 or Pantoea spp. 2 Escherich R 12 Escherichia coli l or Cronobacter sakazakii Cronobacter sakazakii SXT, sulfamethoxazole KAN, kanamycin STR, streptomycin: GEN, gentamycin: S, sensitive:R, resistant I, inter mediate 表4“人体肠道杆菌的分离与鉴定”综合性实验学生操作分析 Tab 4 Assessment of students'operation on comprehensive experiment"Isolation and identification of the enterobacterial strains from human feces Probability of No. Experimental procedure P Cause of failure success 1 Plate streaking Getting a single colony 2 Gram staining Gram negative bacilli Unsuccessful discoloration 3 Liquid culture inoculation 4 Setting, reading and Bacterial identification API 1OS strip enables the identification interpreting API lOS strip and some species are not included in the database 5 Bacterial genome preparation PCR template No products due to unskilled impro ation or careless operation of genomic DNA 6 16S rRNA amplificatio Bacterial identification 7 PCR fragment purification Bacterial identification 80.6 No products due to improper operation of DNA fragment purification TA cloning 8 16S rRNA sequence analysis Bacterial identification 9 Antimicrobial susceptibility Measurement of 100 testing inhibition验效果 ，本研究在实验课结束后针对教学对象 ——— 复旦大学临床医学专业六年制 2 个年级（2011 级和 2012 级）共 36 名同学进行问卷调查和统计分析 ，结 果见表 5 。 表 3 API 10S与 16S rRNA 鉴定及药敏试验结果 Tab ．3 Identification and antimicrobial susceptibility testing results No ． API 10S identification 16S rRNA identification Antimicrobial susceptibility testing SXT KAN STR GEN 1 Escherichia coli 1 Escherichia coli S S S I 2 Escherichia coli 1 Escherichia coli S S I S 3 Serratia odorifera or Escherichia coli 1 Escherichia coli R I I R 照 4 Escherichia coli 1 Escherichia coli I S S S 5 Escherichia coli 1 Escherichia coli R S I R 6 Escherichia coli 1 Cronobacter sakazakii S S S I 7 Serratia odorifera or Escherichia coli 1 Escherichia coli I I I R 照 8 Escherichia coli or Shigella spp ． Escherichia coli R I I R 9 Escherichia coli or Shigella spp ． Escherichia coli R S S I 10 Escherichia coli 2 or Pantoea spp ．2 Escherichia coli S I S R y6 11 Klebsiella pneumoniae ssp ． pneumoniae Klebsiella pneumoniae R I I R 6 12 Escherichia coli 1 or Cronobacter sakazakii Cronobacter sakazakii R I I R 6 SXT ，sulfamethoxazole ；KAN ，kanamycin ；STR ，streptomycin ；GEN ，gentamycin ；S ，sensitive ；R ，resistant ；I ，intermediate ． 表 4 “人体肠道杆菌的分离与鉴定”综合性实验学生操作分析 Tab ．4 Assessment of students’ operation on comprehensive experiment “Isolation and identification of the enterobacterial strains from human feces” No ． Experimental procedure Purpose Probability of success （％ ） Cause of failure 1 Plate streaking Getting a single colony 100 Non 悙 2 Gram staining Gram negative bacilli 89 ．9 Unsuccessful discoloration K 3 Liquid culture inoculation Cell enrichment 100 Non 悙 4 Setting ，reading and interpreting API 10S strip Bacterial identification 50 API 10S strip enables the identification y of 400 species of enterobacteriaceae ， and some species are not included in the database 5 Bacterial genome preparation PCR template preparation 77 ．8 No products due to unskilled ，improper K or careless operation of genomic DNA extraction 6 16S rRNA amplification Bacterial identification 100 Non 悙 7 PCR fragment purification Bacterial identification 80 ．6 No products due to improper operation K of DNA fragment purification TA cloning Bacterial identification 100 Non 悙 8 16S rRNA sequence analysis Bacterial identification 100 Non 悙 9 Antimicrobial susceptibility testing Measurement of inhibition zone 100 Non 悙 · 368 · 微生物与感染 Journal of Microbes and Infections ，December 25 ，2015 ，10（6） ：365‐370