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Name Section Question 2 tinued In the table below, a()indicates that the diploid created grows on minimal media, a(-) indicates that the diploid fails to grow on minimal media Im1 m2m3 m4 7m8m9m10 3 m4+ m++++++++ + n10|+ ii)Assign the mutants 1-10 into complementation groups e) You also characterize your mutants based upon which of the intermediates accumulates lates x m 3: accumulates z m4 accumulates pep m5: accumulates y m6: accumulates w Based on the above data, you predict that m1 has a mutation ir ge m2 has a mutation in gene iii) m3 has a mutation in ge iv) m4 has a mutation in gene v) m5 has a mutation in gene 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 Question 2, continued In the table below, a (+) indicates that the diploid created grows on minimal media, a (-) indicates that the diploid fails to grow on minimal media. m1 m2 m3 m4 m5 m6 m7 m8 m9 m10 m1 - - + + + + - + + + m2 - - + + + + - + + + m3 + + - + + + + + + + m4 + + + - + + + + + - m5 + + + + - + + + - + m6 + + + + + - + - + + m7 - - + + + + - + + + m8 + + + + + - + - + + m9 + + + + - + + + - + m10 + + + - + + + + + - ii) Assign the mutants 1-10 into complementation groups. e) You also characterize your mutants based upon which of the intermediates accumulates m2: accumulates X m3: accumulates Z m4: accumulates PEP m5: accumulates Y m6: accumulates W Based on the above data, you predict that i) m1 has a mutation in gene______ ii) m2 has a mutation in gene______ iii) m3 has a mutation in gene______ iv) m4 has a mutation in gene______ v) m5 has a mutation in gene______
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