Name Section FIGURE 1: plate plate 2 plate 3 plate plate 5 minimal medium+phe a) Plate l is the original rich medium plate that the mutagenized cells were grown on. It is standard practice for the last plate in a series of replica plates to have the same growth medium as the original plate. What purpose might this serve? b)You obtain some phe" colonies in this manner. Identify the colonies from FIGURE 1 that are c) You notice that some colonies do not grow on plates containing minimal medium phenylalanine(plate 2). Identify these colonies and give one possible explanation for the growth behavior of these colonies d)This strategy for isolating phe" mutants works well so you repeat the mutagenesis and replica plating experiment to isolate more phe" mutants. You then perform a complementation test on these phe- mutants i)What is a complementation test? ii)What is the purpose of a complementation test? 7012Fall2003Name:___________________________________ Section:_____ 7.012 Fall 2003 FIGURE 1: A B C D E F G H X Y A F A B F A C E F G H Y A B C D E F G H X Y plate 1 plate 2 plate 3 plate 4 plate 5 rich medium minimal medium + phe minimal medium minimal medium + PEP rich medium a) Plate 1 is the original rich medium plate that the mutagenized cells were grown on. It is standard practice for the last plate in a series of replica plates to have the same growth medium as the original plate. What purpose might this serve? b) You obtain some phe- colonies in this manner. Identify the colonies from FIGURE 1 that are phe￾c) You notice that some colonies do not grow on plates containing minimal medium + phenylalanine (plate 2). Identify these colonies and give one possible explanation for the growth behavior of these colonies. d) This strategy for isolating phe- mutants works well so you repeat the mutagenesis and replica plating experiment to isolate more phe- mutants. You then perform a complementation test on these phe- mutants. i) What is a complementation test? ii) What is the purpose of a complementation test?