GUIDE TO PAM I Pesticide Analytical Manual Vol I Regulatory Operations Chapter l provides information and directions that reflect FDA regu latory policy. PAM I is not the authoritative source for publication of FDA policy, but policy decisions that directly affect the application of Chapter a pesticide analytical methodology are included here as a service to the manual user Chacter s Chapter 2 is a collection of data and directions on ariety of unrelated topics, each of which pro. vides background information needed to perform methods of Chapters 3 and 4 Where information in Chapters I and 2 appears to overlap (e.g, Sec- tions 102, Preparation of Analytical Samples; and Chapter 3 203, Equipment and Procedures for Comminuting) the material in Chapter I reflects the agency policy that must be followed for enforcement of regula tions, and Chapter 2 provides information and hints found useful by FDa pesticide chemists Chapter 3 includes multiclass multiresidue methods(MRMs), those hat are capable of determining pesticide residues of many chemical types. The actual number and identity of the residues determinable by the methods are usually dependent on the number and variety of different determinative steps used to examine the extract. Each method in this chapter is pi s of modules fo the extraction, cleanup, and determinative steps; a complete method is defined as a combination of one or more modules from each step. Complete methods that have been validated in interlaboratory studies, including collaborative studies performed under the aus- pices of AOAC International, are listed; these are sanctioned for use in regulatory analyses. Other combinations of modules must be treated as experimental methodology, additional supporting data for the validity of the analysis are required when such combinations are used in regulatory analyses vo.941(1 2905a(6Transmittal No. 94-1 (1/94) x Form FDA 2905a (6/92) GUIDE TO PAM I Pesticide Analytical Manual Vol. I Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC Chapter 1 provides information and directions that reflect FDA regu￾latory policy. PAM I is not the authoritative source for publication of FDA policy, but policy decisions that directly affect the application of pesticide analytical methodology are included here as a service to the manual user. Chapter 3 includes multiclass multiresidue methods (MRMs), those that are capable of determining pesticide residues of many chemical types. The actual number and identity of the residues determinable by the methods are usually dependent on the number and variety of different determinative steps used to examine the extract. Each method in this chapter is presented as a series of modules for the extraction, cleanup, and determinative steps; a complete method is defined as a combination of one or more modules from each step. Complete methods that have been validated in interlaboratory studies, including collaborative studies performed under the aus￾pices of AOAC International, are listed; these are sanctioned for use in regulatory analyses. Other combinations of modules must be treated as experimental methodology; additional supporting data for the validity of the analysis are required when such combinations are used in regulatory analyses. Chapter 2 is a collection of data and directions on a variety of unrelated topics, each of which pro￾vides background information needed to perform methods of Chapters 3 and 4. Where information in Chapters 1 and 2 appears to overlap (e.g., Sec￾tions 102, Preparation of Analytical Samples; and 203, Equipment and Procedures for Comminuting), the material in Chapter 1 reflects the agency policy that must be followed for enforcement of regula￾tions, and Chapter 2 provides information and hints found useful by FDA pesticide chemists