Pesticide Analytical Manual Vol. I GUIDE TO PAM Chapter 4 includes descriptions of selective MRMS, i.e., methods designed to determine a limited number of residues related by chemical structure. Selective MRMs target residues that are not amenable to determination by the multiclass MRMs of Chapter 3. In actual practice, FDA laboratories often examine an extract from a multiclass MRM with determinative step(s) from selec- tive mRMs to broaden the scope of the analysis when such uses Cheater 3 Chapter 4 have been validated in interlaboratory studies, the determina- MRM e steps are included as modules in Chapter 3 methodology When no interlaboratory validation has occurred, such combi nations are treated as experimental methodology, with the same requirements noted above for use in regulatory analyses Tables of data are provided for each method in Chapters 3 and 4 to describe the analytical behavior of each chemical tested through the method. Data are available only for methods sanctioned for use by virtue of previous interlaboratory studies Chapter 5, GLC, and Chapter 6, HPLC, provide background information about the two major determinative steps used in MRMs. Basic information about the is for lm instruments based on experiences in FDA laboratories Manual users attempting to employ the determinative steps defined in Chapters 3 and 4 may need to refer to Chapters 5 and 6 for additional information and advice Chapter 3 GLC Appendix I combines information on GlC behavior of particular chemicals with re eresos thheachweric astar ou ghevelapt esicm ethers a ppeadioi detineothest ps continuing testingxi Transmittal No. 94-1 (1/94) Form FDA 2905a (6/92) Pesticide Analytical Manual Vol. I GUIDE TO PAM I Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC Chapter 4 includes descriptions of selective MRMs, i.e., methods designed to determine a limited number of residues related by chemical structure. Selective MRMs target residues that are not amenable to determination by the multiclass MRMs of Chapter 3. In actual practice, FDA laboratories often examine an extract from a multiclass MRM with determinative step(s) from selec￾tive MRMs to broaden the scope of the analysis. When such uses have been validated in interlaboratory studies, the determina￾tive steps are included as modules in Chapter 3 methodology. When no interlaboratory validation has occurred, such combi￾nations are treated as experimental methodology, with the same requirements noted above for use in regulatory analyses. Tables of data are provided for each method in Chapters 3 and 4 to describe the analytical behavior of each chemical tested through the method. Data are available only for methods sanctioned for use by virtue of previous interlaboratory studies. Chapter 5, GLC, and Chapter 6, HPLC, provide background information about the two major determinative steps used in MRMs. Basic information about the techniques is included, as well as specific directions for implementing use of the instruments based on experiences in FDA laboratories. Manual users attempting to employ the determinative steps defined in Chapters 3 and 4 may need to refer to Chapters 5 and 6 for additional information and advice. Appendix I combines information on GLC behavior of particular chemicals with recoveries of the chemicals through Chapter 3 methods. Appendix II defines the steps of protocols that were used to develop such method behavior data, for use in continuing testing. Chapter 1 Regulatory Operations Chapter 2 General Analytical Operations and Information Chapter 3 Multiclass MRMs Chapter 5 GLC Chapter 4 Selective MRMs Chapter 6 HPLC