Measuring Km and Vmax z Curve-fitting algorithms can be used to determine Km and Vmax from v vs. [S] plots z Michaelis-Menton equation can be rearranged to the “double reciprocal” plot and Km and Vmax can be graphically determinedMeasuring Km and Vmax z Curve-fitting algorithms can be used to determine Km and Vmax from v vs. [S] plots z Michaelis-Menton equation can be rearranged to the “double reciprocal” plot and Km and Vmax can be graphically determined