Eadie-Hofstee Plot V 0 V 0/[S] Vmax/K M Vmax Slope = -1/K M V 0/[S] = Vmax/ KM – V 0/ K MEadie-Hofstee Plot V 0 V 0/[S] Vmax/K M Vmax Slope = -1/K M V 0/[S] = Vmax/ KM – V 0/ K M