258 Research Progress on the Assembly of Human Papillomavirus Virus-like Particles SHI Jingjie, SONG Shuo, WANG Daning, LI Zhihai, XIA Ningshao. 2, LI Shaowei.2 (1. School of Life Sciences, National Institute of Diagnostics and Vaccine Development of Infectious Diseases, Xiamen University, Xiamen 361102, China 2. School of Public Health, State Key Laboratory of Molecular vaccinology and Molecular Diagnostics, Xiamen University, Xiamen 361102, china Abstract: Virus-like particles(VLPs) have been considered to be "ideal"vaccine candidates in recent years owing to their excellent immunogenicity and safety. VlPs cannot replicate in vivo because they are hollow and lack a viral genome, but they can stimulate the immune system to produce neutralizing antibodies be- cause they mimic the morphology and conformation of native viruses. Human papillomavirus virus (HPV) prophylactic vaccines have been commercialized worldwide: Cervarix( GlaxoSmithKline) and Gardasil ( Merck)are based on virus-like particles. Therefore, study of the assembly of HPv vLPs is important for the research and development of vaccines. We describe progress in the assembly of HPv virus-like partr cles in the past decade to aid development of hpv vaccines as well as genetically engineered vaccines of Key words: Human papillomavirus( HPV); Major capsid protein Ll: Virus-like particles(VLPs); Vaccine; Assembly Funding: National Natural Science Foundation of China(31670935): Research on assembly mechanism and cross-type immunogenicity of Hu man papillomavirus virus-like particles heterozygous for different types of pentamer. National Natural Science Foundation of china (81701637): Characteristics and preliminary localization of the cross-teutralizing epitopes of Human papillomavirus types 16, 31, 33 and 58: Natural Science Foundation of Fujian Province(2017J07005): Research on mechanism and structural basis of neutralization and hetero- CorrespondingauthorLIShaowei,Enail:shaowei@rmu.edu.cn 21994-2018ChinaAcademicJOurnalElectronicpUblishingHouse.Allrightsreservedhttp:/www.cnki.netＲｅｓｅａｒｃｈＰｒｏｇｒｅｓｓｏｎｔｈｅＡｓｓｅｍｂｌｙｏｆＨｕｍａｎＰａｐｉｌｌｏｍａｖｉｒｕｓＶｉｒｕｓ－ｌｉｋｅＰａｒｔｉｃｌｅｓ ＳＨＩＪｉｎｇｊｉｅ１，ＳＯＮＧＳｈｕｏ１，ＷＡＮＧＤａｎｉｎｇ２，ＬＩＺｈｉｈａｉ１，ＸＩＡ Ｎｉｎｇｓｈａｏ１，２，ＬＩＳｈａｏｗｅｉ１，２＊ （１．ＳｃｈｏｏｌｏｆＬｉｆｅＳｃｉｅｎｃｅｓ，ＮａｔｉｏｎａｌＩｎｓｔｉｔｕｔｅｏｆＤｉａｇｎｏｓｔｉｃｓａｎｄＶａｃｃｉｎｅＤｅｖｅｌｏｐｍｅｎｔ ｏｆＩｎｆｅｃｔｉｏｕｓＤｉｓｅａｓｅｓ，ＸｉａｍｅｎＵｎｉｖｅｒｓｉｔｙ，Ｘｉａｍｅｎ３６１１０２，Ｃｈｉｎａ； ２．ＳｃｈｏｏｌｏｆＰｕｂｌｉｃＨｅａｌｔｈ，ＳｔａｔｅＫｅｙＬａｂｏｒａｔｏｒｙｏｆＭｏｌｅｃｕｌａｒＶａｃｃｉｎｏｌｏｇｙ ａｎｄＭｏｌｅｃｕｌａｒＤｉａｇｎｏｓｔｉｃｓ，ＸｉａｍｅｎＵｎｉｖｅｒｓｉｔｙ，Ｘｉａｍｅｎ３６１１０２，Ｃｈｉｎａ） Ａｂｓｔｒａｃｔ：Ｖｉｒｕｓ－ｌｉｋｅｐａｒｔｉｃｌｅｓ（ＶＬＰｓ）ｈａｖｅｂｅｅｎｃｏｎｓｉｄｅｒｅｄｔｏｂｅ“ｉｄｅａｌ”ｖａｃｃｉｎｅｃａｎｄｉｄａｔｅｓｉｎｒｅｃｅｎｔｙｅａｒｓ ｏｗｉｎｇｔｏｔｈｅｉｒｅｘｃｅｌｌｅｎｔｉｍｍｕｎｏｇｅｎｉｃｉｔｙａｎｄｓａｆｅｔｙ．ＶＬＰｓｃａｎｎｏｔｒｅｐｌｉｃａｔｅｉｎｖｉｖｏｂｅｃａｕｓｅｔｈｅｙａｒｅｈｏｌｌｏｗ ａｎｄｌａｃｋａｖｉｒａｌｇｅｎｏｍｅ，ｂｕｔｔｈｅｙｃａｎｓｔｉｍｕｌａｔｅｔｈｅｉｍｍｕｎｅｓｙｓｔｅｍｔｏｐｒｏｄｕｃｅｎｅｕｔｒａｌｉｚｉｎｇａｎｔｉｂｏｄｉｅｓｂｅ－ ｃａｕｓｅｔｈｅｙｍｉｍｉｃｔｈｅｍｏｒｐｈｏｌｏｇｙａｎｄｃｏｎｆｏｒｍａｔｉｏｎｏｆｎａｔｉｖｅｖｉｒｕｓｅｓ．Ｈｕｍａｎｐａｐｉｌｌｏｍａｖｉｒｕｓｖｉｒｕｓ（ＨＰＶ） ｐｒｏｐｈｙｌａｃｔｉｃｖａｃｃｉｎｅｓｈａｖｅｂｅｅｎｃｏｍｍｅｒｃｉａｌｉｚｅｄｗｏｒｌｄｗｉｄｅ：Ｃｅｒｖａｒｉｘ （ＧｌａｘｏＳｍｉｔｈＫｌｉｎｅ）ａｎｄＧａｒｄａｓｉｌ （Ｍｅｒｃｋ）ａｒｅｂａｓｅｄｏｎｖｉｒｕｓ－ｌｉｋｅｐａｒｔｉｃｌｅｓ．Ｔｈｅｒｅｆｏｒｅ，ｓｔｕｄｙｏｆｔｈｅａｓｓｅｍｂｌｙｏｆＨＰＶＶＬＰｓｉｓｉｍｐｏｒｔａｎｔｆｏｒ ｔｈｅｒｅｓｅａｒｃｈａｎｄｄｅｖｅｌｏｐｍｅｎｔｏｆｖａｃｃｉｎｅｓ．ＷｅｄｅｓｃｒｉｂｅｐｒｏｇｒｅｓｓｉｎｔｈｅａｓｓｅｍｂｌｙｏｆＨＰＶｖｉｒｕｓ－ｌｉｋｅｐａｒｔｉ－ ｃｌｅｓｉｎｔｈｅｐａｓｔｄｅｃａｄｅｔｏａｉｄｄｅｖｅｌｏｐｍｅｎｔｏｆＨＰＶｖａｃｃｉｎｅｓａｓｗｅｌｌａｓｇｅｎｅｔｉｃａｌｌｙｅｎｇｉｎｅｅｒｅｄｖａｃｃｉｎｅｓｏｆ ｏｔｈｅｒｖｉｒｕｓｅｓ． Ｋｅｙｗｏｒｄｓ：Ｈｕｍａｎｐａｐｉｌｌｏｍａｖｉｒｕｓ（ＨＰＶ）；ＭａｊｏｒｃａｐｓｉｄｐｒｏｔｅｉｎＬ１；Ｖｉｒｕｓ－ｌｉｋｅｐａｒｔｉｃｌｅｓ（ＶＬＰｓ）；Ｖａｃｃｉｎｅ； Ａｓｓｅｍｂｌｙ Ｆｕｎｄｉｎｇ：ＮａｔｉｏｎａｌＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆＣｈｉｎａ（３１６７０９３５）；Ｒｅｓｅａｒｃｈｏｎａｓｓｅｍｂｌｙｍｅｃｈａｎｉｓｍａｎｄｃｒｏｓｓ－ｔｙｐｅｉｍｍｕｎｏｇｅｎｉｃｉｔｙｏｆＨｕ－ ｍａｎｐａｐｉｌｌｏｍａｖｉｒｕｓｖｉｒｕｓ－ｌｉｋｅｐａｒｔｉｃｌｅｓｈｅｔｅｒｏｚｙｇｏｕｓｆｏｒｄｉｆｆｅｒｅｎｔｔｙｐｅｓｏｆ ｐｅｎｔａｍｅｒ．ＮａｔｉｏｎａｌＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆ Ｃｈｉｎａ （８１７０１６３７）；Ｃｈａｒａｃｔｅｒｉｓｔｉｃｓａｎｄｐｒｅｌｉｍｉｎａｒｙｌｏｃａｌｉｚａｔｉｏｎｏｆｔｈｅｃｒｏｓｓ－ｎｅｕｔｒａｌｉｚｉｎｇｅｐｉｔｏｐｅｓｏｆ Ｈｕｍａｎｐａｐｉｌｌｏｍａｖｉｒｕｓｔｙｐｅｓ１６，３１，３３ａｎｄ ５８；ＮａｔｕｒａｌＳｃｉｅｎｃｅＦｏｕｎｄａｔｉｏｎｏｆＦｕｊｉａｎＰｒｏｖｉｎｃｅ（２０１７Ｊ０７００５）；Ｒｅｓｅａｒｃｈｏｎｍｅｃｈａｎｉｓｍａｎｄｓｔｒｕｃｔｕｒａｌｂａｓｉｓｏｆｎｅｕｔｒａｌｉｚａｔｉｏｎａｎｄｈｅｔｅｒｏ－ ｇｅｎｅｉｔｙｏｆＨｅｐａｔｉｔｅｓＥｖｉｒｕｓ． ＊Ｃｏｒｒｅｓｐｏｎｄｉｎｇａｕｔｈｏｒ：ＬＩＳｈａｏｗｅｉ，Ｅ－ｍａｉｌ：ｓｈａｏｗｅｉ＠ｘｍｕ．ｅｄｕ．ｃｎ · ８５２ · 病 毒 学 报 ３４卷