Chapter 5 Exploring Genes and Genomes 4 5 Reagents necessary for sequencing by chain termination include A)template DNA,deoxyribonucleoside triphosphates(dNTPs),primer,dideoxy nucleotide analogs,DNA polymerase,and radioactive probe. B) template DNA,deoxyribonucleoside triphosphates(dNTPs),primer,dideoxy nucleotide analogs,and DNA polymerase. C) template DNA,deoxyribonucleoside triphosphates(dNTPs),primer,dideoxy nucleotide analogs,RNA polymerase. D) All of the above. E) None of the above Ans:B Section:5.1 6 The first three bases of the 6-base recognition cleavage site of HindllI are AAG.What is the complete sequence of this 6 bp site? A)AAGAAG D) AAGCUU B)AAGCTT E) None of the above C)AAGGAA Ans:B Section:5.1 7 Plasmids used in recombinant DNA technology typically A) possess a gene for antibiotic resistance. B) replicate independently of the host genome. C) are circular double stranded molecules. D) all of the above E)a and b. Ans:D Section:5.2 8 A polylinker site contains A) many closely spaced restriction enzyme sites. B) links for antibiotic resistance. C) sequences allowing linkage to mRNA. D) All of the above. E) None of the above. Ans:A Section:5.2 9 Why are met and trp often used to design DNA probes from amino acid sequences? A)They are not degenerate and have single codons. B) Met is the first amino acid in the protein chain. C) Both are used often in proteins. D) All of the above. E) None of the above. Ans:A Section:5.2 10 For identification of a gene,against which strand of DNA must the probe complement:the top or bottom strand? A)either strand D only the template strand B)both strands E) None of the above. C)only the coding strand Ans:A Section:5.2Chapter 5 Exploring Genes and Genomes 4 5 Reagents necessary for sequencing by chain termination include A) template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxy nucleotide analogs, DNA polymerase, and radioactive probe. B) template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxy nucleotide analogs, and DNA polymerase. C) template DNA, deoxyribonucleoside triphosphates (dNTPs), primer, dideoxy nucleotide analogs, RNA polymerase. D) All of the above. E) None of the above Ans: B Section: 5.1 6 The first three bases of the 6-base recognition cleavage site of HindIII are AAG. What is the complete sequence of this 6 bp site? A) AAGAAG D) AAGCUU B) AAGCTT E) None of the above. C) AAGGAA Ans: B Section: 5.1 7 Plasmids used in recombinant DNA technology typically A) possess a gene for antibiotic resistance. B) replicate independently of the host genome. C) are circular double stranded molecules. D) all of the above. E) a and b. Ans: D Section: 5.2 8 A polylinker site contains A) many closely spaced restriction enzyme sites. B) links for antibiotic resistance. C) sequences allowing linkage to mRNA. D) All of the above. E) None of the above. Ans: A Section: 5.2 9 Why are met and trp often used to design DNA probes from amino acid sequences? A) They are not degenerate and have single codons. B) Met is the first amino acid in the protein chain. C) Both are used often in proteins. D) All of the above. E) None of the above. Ans: A Section: 5.2 10 For identification of a gene, against which strand of DNA must the probe complement: the top or bottom strand? A) either strand D) only the template strand B) both strands E) None of the above. C) only the coding strand Ans: A Section: 5.2