Chapter 5 Exploring Genes and Genomes 3 6 PCR is the abbreviation for which is an in-vitro technique used to make multiple copies of a DNA molecule. Ans:Polymerase Chain Reaction Section:5.1 7 Bacterial plasmid DNA and bacteriophage DNA are commonly used to introduce foreign DNA into a bacterium. Ans:vectors Section:5.1 8 The enzyme can be used to add nucleotides to the 3'end of DNA Ans:terminal transferase Section:5.4 9 Complimentary DNA(cDNA)is formed by the action of reverse transcriptase on Ans:mRNA Section:5.4 10 DNA fragments can be visualized in polyacrylamide gel electrophoresis by staining with which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light. Ans:ethidium bromide Section:5.1 Multiple Choice Questions 1 The biological role of restriction enzymes in bacteria is to A)repair DNA. D) All of the above. B) induce DNA crossover. E) None of the above. C) cleave foreign DNA. Ans:C Section:5.1 2 Which of the following DNA sequences contains a 4-8 base palindromic site?(Note:Only one strand is shown. A) CAGTCC D GAGAGAGA B) GCATCC E) GCATATGC C) CGATTAGC Ans:E Section:5.1 3 What do Southern,Northern,and Western blots detect,respectively? A) DNA,RNA,and protein D) protein,DNA,and RNA B) DNA,protein,and RNA E) RNA,protein,and DNA C)RNA,DNA,and protein Ans:A Section:5.1 4 The specificity or strigency of a PCR reaction can be controlled by altering the reaction A)volume. D)all of the above. B)target sequence. E) None of the above. C)temperature and salt concentration. Ans:C Section:5.1Chapter 5 Exploring Genes and Genomes 3 6 PCR is the abbreviation for ____________________________, which is an in-vitro technique used to make multiple copies of a DNA molecule. Ans: Polymerase Chain Reaction Section: 5.1 7 Bacterial plasmid DNA and bacteriophage DNA are commonly used ______________ to introduce foreign DNA into a bacterium. Ans: vectors Section: 5.1 8 The enzyme _________________ can be used to add nucleotides to the 3' end of DNA. Ans: terminal transferase Section: 5.4 9 Complimentary DNA (cDNA) is formed by the action of reverse transcriptase on ___________. Ans: mRNA Section: 5.4 10 DNA fragments can be visualized in polyacrylamide gel electrophoresis by staining with _______________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light. Ans: ethidium bromide Section: 5.1 Multiple Choice Questions 1 The biological role of restriction enzymes in bacteria is to A) repair DNA. D) All of the above. B) induce DNA crossover. E) None of the above. C) cleave foreign DNA. Ans: C Section: 5.1 2 Which of the following DNA sequences contains a 48 base palindromic site? (Note: Only one strand is shown.) A) CAGTCC D) GAGAGAGA B) GCATCC E) GCATATGC C) CGATTAGC Ans: E Section: 5.1 3 What do Southern, Northern, and Western blots detect, respectively? A) DNA, RNA, and protein D) protein, DNA, and RNA B) DNA, protein, and RNA E) RNA, protein, and DNA C) RNA, DNA, and protein Ans: A Section: 5.1 4 The specificity or strigency of a PCR reaction can be controlled by altering the reaction A) volume. D) all of the above. B) target sequence. E) None of the above. C) temperature and salt concentration. Ans: C Section: 5.1