Chapter 5 Exploring Genes and Genomes 2 6 Won the Nobel prize for discovery of the polymerase chain reaction. Ans:I Section:5.1 7 A plasmid is an example of a common Ans:g Section:5.2 P A plasmid vector that contains the B-galactosidase gene and is useful for screening cells with recombined DNA. Ans:c Section:5.2 9 The enzyme used to create DNA from RNA Ans:j Section:5.4 10 The type of vector used for synthesis of protein. Ans:d Section:5.4 Fill in the Blank Questions 1 The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is Ans:DNA ligase Section:5.1 cleave DNA at sites with inverted repeat sequences referred to as palendromic sequences. Ans:Restriction endonucleases Section:5.1 3 Complementary,single-strand overhangs that are produced by some restriction endonucleases are referred to as Ans:sticky ends or cohesive ends Section:5.2 4 The Sanger technique for sequencing DNA involves the use of nucleotide analogs that terminate chain elongation. Ans:2,3'-dideoxy Section:5.1 5 In solid-phase synthesis of oligonucleotides,the 2'deoxyribonucleotide-3'-phosphoramidite is added to the end of the growing oligonucleotide. Ans:5'Section:5.1Chapter 5 Exploring Genes and Genomes 2 6 ____________ Won the Nobel prize for discovery of the polymerase chain reaction. Ans: l Section: 5.1 7 A plasmid is an example of a common ____________. Ans: g Section: 5.2 8 ____________ A plasmid vector that contains the β-galactosidase gene and is useful for screening cells with recombined DNA. Ans: c Section: 5.2 9 ____________ The enzyme used to create DNA from RNA. Ans: j Section: 5.4 10 ____________ The type of vector used for synthesis of protein. Ans: d Section: 5.4 Fill in the Blank Questions 1 The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is __________________. Ans: DNA ligase Section: 5.1 2 ___________________________ cleave DNA at sites with inverted repeat sequences referred to as palendromic sequences. Ans: Restriction endonucleases Section: 5.1 3 Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as ___________________. Ans: sticky ends or cohesive ends Section: 5.2 4 The Sanger technique for sequencing DNA involves the use of __________________ nucleotide analogs that terminate chain elongation. Ans: 2',3'-dideoxy Section: 5.1 5 In solid-phase synthesis of oligonucleotides, the 2'deoxyribonucleotide-3'-phosphoramidite is added to the _______ end of the growing oligonucleotide. Ans: 5' Section: 5.1