The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class.The teacher should display the basic experimental steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价Evaluation: The teacher will evaluate the experimental procedure done by the students on-site.The students will finish the experimental report.The students should summarize the experimental result and attach necessary documents from their lab experiment.The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 6 Quantitative Determination Of The Serum Protein With Cellulose Acetate Membrane Electrophoresis 4.教学目标Aims: (1)Comprehend the method of electrophoresis. (2) Familiar with the percentage values of human serum protein. (3)Master the method of cellulose acetate membrane electrophoresis to separate and identify serum protein. 2.教学重难点Key points: Principles to separate serum proteins with cellulose acetate membrane electrophoresis 3.教学内容Content: The isoelectric point (PI)of various kinds of serum proteins are generally lower than 7.0,in alkaline buffer solution(PH 8.6),serum proteins are negatively charged, they will migrate toward anode in an electric field.The rate of migration will depend on the strength of their net surface charges,shape and size of the proteins.Because the PI of the different serum proteins are different,so their net surface charges are different.Furthermore,the size shape are distinct,consequently,the rate of migration is different too. 4.教学方法Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class.The teacher should display the basic experimentalThe teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class. The teacher should display the basic experimental steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价 Evaluation： The teacher will evaluate the experimental procedure done by the students on-site. The students will finish the experimental report. The students should summarize the experimental result and attach necessary documents from their lab experiment. The students should also discuss the reason for experimental failures. The teacher will evaluate the report according to their experimental result and discussion. Experiment 6 Quantitative Determination Of The Serum Protein With Cellulose Acetate Membrane Electrophoresis 4. 教学目标 Aims: （1） Comprehend the method of electrophoresis. （2） Familiar with the percentage values of human serum protein. （3） Master the method of cellulose acetate membrane electrophoresis to separate and identify serum protein. 2.教学重难点 Key points: Principles to separate serum proteins with cellulose acetate membrane electrophoresis 3.教学内容 Content: The isoelectric point (PI) of various kinds of serum proteins are generally lower than 7.0, in alkaline buffer solution (PH 8.6), serum proteins are negatively charged, they will migrate toward anode in an electric field. The rate of migration will depend on the strength of their net surface charges、shape and size of the proteins. Because the PI of the different serum proteins are different, so their net surface charges are different. Furthermore, the size 、shape are distinct, consequently, the rate of migration is different too. 4.教学方法 Teaching Methods: The teacher should recapitulate the key concept of the corresponding chapter taught in the theoretical class. The teacher should display the basic experimental