steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价Evaluation: (1)The teacher should supervise students during the whole process of experiment and record if the students have attentively and successfully finished the experiment. (2)The students will hand in the experiment report before deadline.In the report,the raw data and the result after calculation should be included and the students should discuss the clinical significance of the result from the experiment.Student should also discuss the reason for experimental failures. Teacher will evaluate the report according to their experimental result and discussion. Experiment 5 Purification of y-globulin From Serum 1.教学目标Aims: (1) Understand the principle of gel filtration, (2) Understand the protein purification method (3)Master gel filtration procedure. 2.教学重难点Key Point: (1) Principle of salting out method for protein purification (2) Principle of gel filtration (3) Packing gel filtration column with Sephorax G25 (4)Protein and NH4S04 detection by ninhydrin solution and Nessler solution. 3.教学内容Content: Separating y-globulin from serum using salting out method using NH,SO,solution. Purify y-globulin by gel filtration and detecting separated protein and NH SO,using ninhydrin solution and Nessler solution.The students will learn to perform salting out and to pack gel filtration column themselves. 4.教学方法Teaching Methods:steps to the students orally and remind students of the key points that determine the successful accomplishment of the experiment. 5.教学评价 Evaluation: （1） The teacher should supervise students during the whole process of experiment and record if the students have attentively and successfully finished the experiment. （2） The students will hand in the experiment report before deadline. In the report, the raw data and the result after calculation should be included and the students should discuss the clinical significance of the result from the experiment. Student should also discuss the reason for experimental failures. Teacher will evaluate the report according to their experimental result and discussion. Experiment 5 Purification of -globulin From Serum 1. 教学目标 Aims: （1） Understand the principle of gel filtration, （2） Understand the protein purification method （3） Master gel filtration procedure. 2. 教学重难点 Key Point: （1） Principle of salting out method for protein purification （2） Principle of gel filtration （3） Packing gel filtration column with Sephorax G25 （4） Protein and NH4SO4 detection by ninhydrin solution and Nessler solution. 3. 教学内容 Content: Separating -globulin from serum using salting out method using NH4SO4 solution. Purify -globulin by gel filtration and detecting separated protein and NH4SO4 using ninhydrin solution and Nessler solution. The students will learn to perform salting out and to pack gel filtration column themselves. 4.教学方法 Teaching Methods: