I.DNA Microarray Let's perform a microarray! 1) Collect Samples. 基因组DNA 基因组DNA 秒照样本 实脸样本 2) Isolate mRNA. 3) Create Labelled DNA. 4)Hybridization. 参照样本标记 实验样本标记 Cy3(级色荧光) 5) Cy5(红色黄光 Microarray Scanner. 6) Analyze Data. 两个样品共同 杂交一张芯片 laser ratio Sample Purification RT Coupling Hybridization Scanning Normalization and washes and analysisLet’s perform a microarray! 1) Collect Samples. 2) Isolate mRNA. 3) Create Labelled DNA. 4) Hybridization. 5) Microarray Scanner. 6) Analyze Data. I. DNA Microarray