傅更洋,等组织工程化神经修复周国神经创伤的应用 Www. cRter.o [6] Kim DH, Connolly SE, Kline DGet al Labeled Schwann [25]Van RL, Bayliss CE, Roncari DA Cytological transplants versus sural nerve grafts in nerve repair. J enzymological characterization of adult human adipocyte Neurosurg.199480(2)254-260 precursors in culture. J Clin Invest. 1976: 58(3): 699-704. [7 Hudson TW, Evans GR, Schmidt CE. Engineering strategies [26 Gimble J, Guilak F Adipose-derived adult stem cells: isolation, for peripheral nerve repair. Orthop Clin North Am characterization, and differentiation potential. Cytotherapy 2000:31(3)485-498 [8] Ringe J, Kaps C, Burmester GR, et al. Stem cells for [27 Strem BM, Hicok KC, Zhu M,et al. Multipotential differentiatio regenerative medicine advances in the engineering of tissues of adipose tissue-derived stem cells. Keio J Med and organs. Naturwissenschaften. 2002: 89(8): 338-351 2005;54(3):132-14 [9] Rodriguez FJ, Verdu E, Ceballos D, et al. Nerve guides [28] De Ugarte DA, Morizono K, Elbarbary A, et al. Comparison of seeded with autologous schwann cells improve nerve regeneration. Exp Neurol. 2000: 161(2): 571-584 marrow Cells Tissues Organs. 2003: 174(3): 101-109. [10 Spierings E, Vleggeert-Lankamp CL, Marani E, et al. [29 Pittenger MF, Mackay AM, Beck SC, et al. Multilineage Allorecognition of artificial nerve guides filed with human potential of adult human mesenchymal stem cells. Science Schwann cells: an in vitro pilot study. Transplantation 1999284(5411):143-147 200069(3)455-456 [30 Aust L, Devlin B, Foster SJ, et al. Yield of human [11] Sheng Z, Fu X, Cai S, et al. Regeneration of functional sweat adipose-derived adult stem cells from liposuction aspirates gland-like structures by transplanted differentiated bone Cytotherapy. 2004: 6(1): 7-14 marrow mesenchymal stem cells. Wound Repair Regen [31 Kingham PJ, Kalbermatten DF, Mahay D,et al 2009:17(3)427-435 Adipose-derived stem cells differentiate into a Schwann cell [12 Oric D, Kajstura J, Chimenti S, et al. Bone marrow cel genotype and promote neurite outgrowth in vitro Exp Neurol. regenerate infarcted myocardium. Nature 2007207(2):267-274 2001410(6829):701-705 [32 Xu Y, Liu L, Li Y,et al. Myelin-forming ability of Schwann [13]Oswald J, Boxberger S, Jorgensen B, et al. Mesenchymal cell-like cells induced from rat adipose-derived stem cells in stem cells can be differentiated into endothelial cells in vitro. Brain Res. 2008: 1239: 49-55 vitro Stem Cells. 2004; 22(3): 377-384 [33 Chi Gf, Kim MR, Kim DW, et al. Schwann cells differentiated [14]Kopen GC, Prockop DJ, Phinney DG Marrow stromal cells from spheroid-forming cells of rat subcutaneous fat tissue migrate throughout forebrain and cerebellum, and they myelinate axons in the spinal cord injury. Exp Neurol differentiate into astrocytes after injection into neonatal mouse 2010222(2):304317 brains. Proc Natl Acad Sci U SA. 1999; 96(19): 10711-10716. [34 di Summa PG, Kalbermatten DF, Pralong E, et al. Long-term in 5 Hofstetter CP, Schwarz EJ, Hess D, et al. Marrow stromal cells vivo regeneration of peripheral nerves through bioengineered form guiding strands in the injured spinal cord and promote erve grafts Neuroscience. 2011: 181: 278-291 covery. Proc Natl Acad Sci U SA. 2002: 99(4): 2199-2204 [35] Bithell A, Williams BP Neural stem cells and cell replacement [16]Jiang Y, Jahagirdar BN, Reinhardt RL, et al. Pluripotency of therapy: making the right cells. Clin Sci (Lond mesenchymal stem cells derived from adult marrow Nature. 2005:108(1):13-22. 02418(6893)41-49 6] Murakami T, Fujimoto Y, Yasunaga Y,et al. Transplanted [17] Tohill M, Mantovani C, Wiberg M, et al. Rat bone marrow neuronal progenitor cells in a peripheral nerve gap promote mesenchymal stem cells express glial markers and stimulate nerve repair. Brain Res. 2003: 974(1-2): 17-24 nerve regeneration. Neurosci Lett. 2004: 362(3): 200-203 [37 Heine W, Conant K, Griffin JW, et al. Transplanted neural stem [18]Choi BH, Zhu SJ, Kim BY, et al. Transplantation of cultured cells promote axonal regeneration through chronically one marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg. 2005; 34(5): 537-542 2004:189(2)231-240. [19 Caddick J, Kingham PJ, Gardiner NJ, et al. Phenotypic and [38 Luis AL, Rodrigues JM, Geuna S, et al. Use of PLGA 90: 10 ional characteristics of differentiated along a Schwann cell lineage. Glia repairing rat sciatic nerve defects. Tissue Eng Part A. 2006;54(8)840-849 2008:14(6):979-993 [20Keilhoff G, Goihl A, Stang F,et al. Peripheral nerve tissue [39 Amado s, Rodrigues JM, Luis AL, et al. Effects of collagen ngineering autologous Schwann cells vs transdifferentiated membranes enriched with in vitro-differentiated n1E-115 cells chymal stem cells Tissue Eng. 2006; 12(6): 1451-146 on rat nerve regeneration after end-to-end repair. J [21] Mahay D, Terenghi G. Shawcross SG Schwann cell mediated Neuroeng Rehabil. 2010, 7: 7. trophic effects by differentiated mesenchymal stem cells. Exp [40] Radtke C, Redeker J, Jokuszies A, et al. In vivo transformation Cell res.2008314(14):2692-2701 of neural stem cells following transplantation in the injure [22] Brohlin M, Mahay D, Novikov LN, et al. Characterisation of nervous system. J Reconstr Microsurg 2010; 26(3): 211-212. human mesenchymal stem cells following differentiation into 1] Amariglio N, Hirshberg a, Schei Schwann cell-like cells. Neurosci Res 2009: 64(1): 41-49 Donor-derived brain tumor following neural stem cell [23 Evans GR Challenges to nerve regeneration. Semin Surg transplantation in an ataxia telangiectasia patient. PLoS Med Oncol.2000;19(3)312318 20096(2)e1000029 [24] Hollenberg CH, Vost A Regulation of DNA synthesis in fat [42] Evans MJ, Kaufman MH Establishment in culture of cells and stromal elements from rat adipose tissue. J Clin ntial cells from nvest.196947(11):2485-2498. 1981292(5819):154156 /SSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 7339 o1994-2013ChinaAcademicJournalElectronicPublishingHouse.Allrightsreservedhttp:/www.cnki.net傅重洋，等. 组织工程化神经修复周围神经创伤的应用 ISSN 2095-4344 CN 21-1581/R CODEN: ZLKHAH 7339 www.CRTER.org [6] Kim DH, Connolly SE, Kline DG,et al. Labeled Schwann cell transplants versus sural nerve grafts in nerve repair. J Neurosurg. 1994;80(2):254-260. [7] Hudson TW, Evans GR, Schmidt CE. Engineering strategies for peripheral nerve repair. Orthop Clin North Am. 2000;31(3):485-498. [8] Ringe J, Kaps C, Burmester GR,et al. Stem cells for regenerative medicine: advances in the engineering of tissues and organs. Naturwissenschaften. 2002;89(8):338-351. [9] Rodríguez FJ, Verdú E, Ceballos D,et al. Nerve guides seeded with autologous schwann cells improve nerve regeneration. Exp Neurol. 2000;161(2):571-584. [10] Spierings E, Vleggeert-Lankamp CL, Marani E,et al. Allorecognition of artificial nerve guides filed with human Schwann cells: an in vitro piloot study. Transplantation. 2000;69(3):455-456. [11] Sheng Z, Fu X, Cai S,et al. Regeneration of functional sweat gland-like structures by transplanted differentiated bone marrow mesenchymal stem cells. Wound Repair Regen. 2009;17(3):427-435. [12] Orlic D, Kajstura J, Chimenti S,et al. Bone marrow cells regenerate infarcted myocardium. Nature. 2001;410(6829):701-705. [13] Oswald J, Boxberger S, Jørgensen B,et al. Mesenchymal stem cells can be differentiated into endothelial cells in vitro.Stem Cells. 2004;22(3):377-384. [14] Kopen GC, Prockop DJ, Phinney DG. Marrow stromal cells migrate throughout forebrain and cerebellum, and they differentiate into astrocytes after injection into neonatal mouse brains. Proc Natl Acad Sci U S A. 1999;96(19):10711-10716. [15] Hofstetter CP, Schwarz EJ, Hess D,et al. Marrow stromal cells form guiding strands in the injured spinal cord and promote recovery. Proc Natl Acad Sci U S A. 2002;99(4):2199-2204. [16] Jiang Y, Jahagirdar BN, Reinhardt RL,et al. Pluripotency of mesenchymal stem cells derived from adult marrow. Nature. 2002;418(6893):41-49. [17] Tohill M, Mantovani C, Wiberg M,et al. Rat bone marrow mesenchymal stem cells express glial markers and stimulate nerve regeneration. Neurosci Lett. 2004;362(3):200-203. [18] Choi BH, Zhu SJ, Kim BY,et al. Transplantation of cultured bone marrow stromal cells to improve peripheral nerve regeneration. Int J Oral Maxillofac Surg. 2005;34(5):537-542. [19] Caddick J, Kingham PJ, Gardiner NJ,et al. Phenotypic and functional characteristics of mesenchymal stem cells differentiated along a Schwann cell lineage.Glia. 2006;54(8):840-849. [20] Keilhoff G, Goihl A, Stang F,et al. Peripheral nerve tissue engineering: autologous Schwann cells vs. transdifferentiated mesenchymal stem cells.Tissue Eng. 2006 ;12(6):1451-1465. [21] Mahay D, Terenghi G, Shawcross SG. Schwann cell mediated trophic effects by differentiated mesenchymal stem cells. Exp Cell Res. 2008;314(14):2692-2701. [22] Brohlin M, Mahay D, Novikov LN,et al. Characterisation of human mesenchymal stem cells following differentiation into Schwann cell-like cells. Neurosci Res. 2009;64(1):41-49. [23] Evans GR. Challenges to nerve regeneration. Semin Surg Oncol. 2000;19(3):312-318. [24] Hollenberg CH, Vost A. Regulation of DNA synthesis in fat cells and stromal elements from rat adipose tissue. J Clin Invest. 1969;47(11):2485-2498. [25] Van RL, Bayliss CE, Roncari DA. Cytological and enzymological characterization of adult human adipocyte precursors in culture. J Clin Invest. 1976;58(3):699-704. [26] Gimble J, Guilak F. Adipose-derived adult stem cells: isolation, characterization, and differentiation potential. Cytotherapy. 2003;5(5):362-369. [27] Strem BM, Hicok KC, Zhu M,et al. Multipotential differentiation of adipose tissue-derived stem cells. Keio J Med. 2005;54(3):132-141. [28] De Ugarte DA, Morizono K, Elbarbary A,et al. Comparison of multi-lineage cells from human adipose tissue and bone marrow. Cells Tissues Organs. 2003;174(3):101-109. [29] Pittenger MF, Mackay AM, Beck SC,et al. Multilineage potential of adult human mesenchymal stem cells. Science. 1999;284(5411):143-147. [30] Aust L, Devlin B, Foster SJ,et al. Yield of human adipose-derived adult stem cells from liposuction aspirates. Cytotherapy. 2004;6(1):7-14. [31] Kingham PJ, Kalbermatten DF, Mahay D,et al. Adipose-derived stem cells differentiate into a Schwann cell phenotype and promote neurite outgrowth in vitro. Exp Neurol. 2007;207(2):267-274. [32] Xu Y, Liu L, Li Y,et al. Myelin-forming ability of Schwann cell-like cells induced from rat adipose-derived stem cells in vitro. Brain Res. 2008;1239:49-55. [33] Chi GF, Kim MR, Kim DW,et al. Schwann cells differentiated from spheroid-forming cells of rat subcutaneous fat tissue myelinate axons in the spinal cord injury. Exp Neurol. 2010;222(2):304-317. [34] di Summa PG, Kalbermatten DF, Pralong E,et al. Long-term in vivo regeneration of peripheral nerves through bioengineered nerve grafts. Neuroscience. 2011;181:278-291. [35] Bithell A, Williams BP. Neural stem cells and cell replacement therapy: making the right cells. Clin Sci (Lond). 2005;108(1):13-22. [36] Murakami T, Fujimoto Y, Yasunaga Y,et al. Transplanted neuronal progenitor cells in a peripheral nerve gap promote nerve repair. Brain Res. 2003;974(1-2):17-24. [37] Heine W, Conant K, Griffin JW,et al. Transplanted neural stem cells promote axonal regeneration through chronically denervated peripheral nerves. Exp Neurol. 2004;189(2):231-240. [38] Luís AL, Rodrigues JM, Geuna S,et al. Use of PLGA 90:10 scaffolds enriched with in vitro-differentiated neural cells for repairing rat sciatic nerve defects.Tissue Eng Part A. 2008;14(6):979-993. [39] Amado S, Rodrigues JM, Luís AL,et al. Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair. J Neuroeng Rehabil. 2010;7:7. [40] Radtke C, Redeker J, Jokuszies A,et al. In vivo transformation of neural stem cells following transplantation in the injured nervous system. J Reconstr Microsurg. 2010;26(3):211-212. [41] Amariglio N, Hirshberg A, Scheithauer BW,et al. Donor-derived brain tumor following neural stem cell transplantation in an ataxia telangiectasia patient. PLoS Med. 2009;6(2):e1000029. [42] Evans MJ, Kaufman MH. Establishment in culture of pluripotential cells from mouse embryos. Nature. 1981;292(5819):154-156