1. Reporter Gene Assay Measures the in vitro level of gene expression or transcriptional activity Transcription is initiated by binding of specific proteins to specific dna promoter regions Reporter assays use a specific DNA promoter fused to a reporter gene(luciferase, SEAP, GFP, etc.) Transcriptional activity of your promoter is measured y reporter expression Department of Biochemistry Molecular Biology eDepartment of Biochemistry & Molecular Biology 7 Measures the in vitro level of gene expression or transcriptional activity Transcription is initiated by binding of specific proteins to specific DNA promoter regions Reporter assays use a specific DNA promoter fused to a reporter gene (luciferase, SEAP, GFP, etc.) Transcriptional activity of your promoter is measured by reporter expression 1. Reporter Gene Assay