Sample Preparation ● Sampling stool samples used in the majority of studies) mucosal biopsy samples(contain large amount of host dna ● nformation recording Where, when, and under which conditions the sampling is made Other factors(freshness, storage) o All sequencing based technologies have an inherent extraction method bias(some microbes are orders of magnitude more difficult to lyse than othersSample Preparation  Sampling  stool samples (used in the majority of studies)  mucosal biopsy samples (contain large amount of host DNA)  Information recording  Where, when, and under which conditions the sampling is made  Other factors (freshness, storage)  All sequencing based technologies have an inherent extraction￾method bias (some microbes are orders of magnitude more difficult to lyse than others)