DNA library preparation 4.5 hours Ligation .Genome fragmented by nebulization Selection .No cloning;no colony (isolate AB picking Emulsion PCR 8 hours Sequencing 7.5 hours .Well diameter:average of 44 um .400,000 reads obtained in parallel .A single cloned amplified sstDNA bead is deposited per well Amplified sstDNA library beads Quality filtered basesRoche/454 FLX Pyrosequencer