Second-generation DNA sequencing technologies Roche/454 FLX Pyrosequencer Illumina/Solexa Genome Analyzer Applied Biosystems SOLiDTM Sequencer Pacific Sequencer HeliScope Single Molecule Sequencer Cost per Cost per 1°error Feature generation Sequencing by synthesis megabase instrument Paired ends? modality Read-length 454 Emulsion PCR Polymerase(pyrosequencing) -$60 $500.000 Yes Indel 250bp Solexa Bridge PCR Polymerase (reversible terminators) -$2 $430.000 Yes Subst. 36bp SOLiD Emulsion PCR Ligase (octamers with two-base encoding) -$2 $591,000 Yes Subst. 35 bp Polonator Emulsion PCR Ligase(nonamers) -$1 $155.000 Yes Subst. 13bp HeliScope Single molecule Polymerase (asynchronous extensions) -$1 $1,350,000 Yes Del 30 bpSecond-generation DNA sequencing technologies  Roche/454 FLX Pyrosequencer  Illumina/Solexa Genome Analyzer  Applied Biosystems SOLiDTM Sequencer  Pacific Sequencer  HeliScope Single Molecule Sequencer