Another mutant cdkY-is a cold-sensitive mutant that arrests at 18C, but grows normally at 25C. cdkY-cells arrest between the s and G2 phases of the cell make cdkX-cdkY-double mutants and perform the following experiments. cycle. You You incubate cdkX-cdkY- double mutants at 36C until all the cells are synchronized and then shift the cells to 18 The cells arrest without dividing You incubate cdkX-cdkY-double mutants at 18C until all until all the cells are synchronized and then shift the cells to 360 The cells divide once and then arrest b)given these experiments you can assume that the protein encoded by the cdk X gene is not required at what phase(s)of the cell? c)In what stage(s)of the cell cycle do you expect the protein encoded by the cdkx gene to act? d)A cell in the process of DNA replication(cell A)is fused with a cell in early G1(cell B).How does the timing of the cell cycle for the cell B nucleus in the fused cell compare to that expected for the cell B nucleus if the cells remained independent? e)What soluble factor(s)are found in cell a but not in cell B(check all that apply)? pRB protein a camP dependent kinase TGFβ a G2 cyclin Human papilloma virus an S-phase cyclin a cyclin dependent kinase an oncogene a g1 cyclin4 Another mutant cdkY- is a cold-sensitive mutant that arrests at 18 °C, but grows normally at 25 °C. cdkY- cells arrest between the S and G2 phases of the cell cycle. You make cdkX- cdkY- double mutants and perform the following experiments. • You incubate cdkX- cdkY- double mutants at 36 °C until all the cells are synchronized and then shift the cells to 18 °. • The cells arrest without dividing. • You incubate cdkX- cdkY- double mutants at 18 °C until all until all the cells are synchronized and then shift the cells to 36 °C. • The cells divide once and then arrest. b) Given these experiments you can assume that the protein encoded by the cdkX gene is not required at what phase(s) of the cell? c) In what stage(s) of the cell cycle do you expect the protein encoded by the cdkX gene to act? d) A cell in the process of DNA replication (cell A) is fused with a cell in early G1 (cell B). How does the timing of the cell cycle for the cell B nucleus in the fused cell compare to that expected for the cell B nucleus if the cells remained independent? e) What soluble factor(s) are found in cell A but not in cell B (check all that apply)? ___ pRB protein ___ a cAMP dependent kinase ___ TGFβ ___ a G2 cyclin ___ Human papilloma virus ___ an S-phase cyclin ___ a cyclin dependent kinase ___ an oncogene ____ a G1 cyclin