MIT Biology Department 7.012: Introductory Biology-Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel 7.012 quiz practice Quiz 3 on Friday, November 12th 10-11 AM Review Session Wednesday 11/10 from 7-9 pm Tutoring Session Thurs. 11/11 from 4-6 pm Final Exam is on Monday, December 13th 9-noon
7.012 Quiz 3 practice Quiz 3 on Friday, November 12th 10 – 11 AM Review Session Wednesday 11/10 from 7-9 pm Tutoring Session Thurs. 11/11 from 4-6 pm MIT Biology Department 7.012: Introductory Biology - Fall 2004 Instructors: Professor Eric Lander, Professor Robert A. Weinberg, Dr. Claudette Gardel
Question 1 A)You infected mice with mouse mammary tumor virus(a retrovirus). After a period of time, most infected mice had developed breast tumors whereas uninfected mice did not. You isolated cell lines from over 50 independent tumors. You demonstrated that all of these lines had virus integrations in the same chromosomal location. Can one conclude that the virus integrates into cellular dNA at only one site? Explain. B)The ras oncogene is involved in a variety of human and animal cancers. DNA was isolated from a number of normal and cancerous tissues -Cellular dna was digested with ecori Digested DNA was separated by gel electrophoresis and transfered to a nitrocellulose membrane The membrane was probed with the radioactive labelled cloned ras DNA and then the membrane was exposed to x-ray film The resulting autoradiograph is shown below 1)white blood cells from a healthy human 2)human lymphoma cells(cancerous) 3)human bladder carcinoma cells(cancerous) 4)human sarcoma cells(cancerous) 5)blood from a healthy mouse 6)mouse myeloma cells(cancerous) 3 10 kb 5 kb 1 kb more than 2 copies/genome 2 copies/genome 1 copies/genome
2 Question 1 A) You infected mice with mouse mammary tumor virus (a retrovirus). After a period of time, most infected mice had developed breast tumors, whereas uninfected mice did not. You isolated cell lines from over 50 independent tumors. You demonstrated that all of these lines had virus integrations in the same chromosomal location. Can one conclude that the virus integrates into cellular DNA at only one site? Explain. B) The ras oncogene is involved in a variety of human and animal cancers. DNA was isolated from a number of normal and cancerous tissues. -Cellular DNA was digested with EcoRI. -Digested DNA was separated by gel electrophoresis and transfered to a nitrocellulose membrane. -The membrane was probed with the radioactive labelled cloned ras DNA and then the membrane was exposed to x-ray film. -The resulting autoradiograph is shown below. 1) white blood cells from a healthy human 2) human lymphoma cells (cancerous) 3) human bladder carcinoma cells (cancerous) 4) human sarcoma cells (cancerous) 5) blood from a healthy mouse 6) mouse myeloma cells (cancerous) 1 2 3 4 5 6 10 kb 5 kb 1 kb more than 2 copies/genome 2 copies/genome 1 copies/genome
Question 1 continued a) How do you explain the presence of sequences complementary to the oncogene in the DNA from healthy human and mouse samples? Why don' t they have cancer? b)Why is the hybridizing band from sample l a different size than that from sample 5? c)For each cancer examined above, based on the autoradiogram, choose the most likely mechanism of transformation and explain your choice 1)point mutation within the gene 2 )chromosomal rearrangement involving the gene 3)gene amplification 4)oncogenic retroviral insertion. Question 2 You are studying the cell cycle in haploid yeast cells. You isolate a cell that is a 25 C, but arrests at 36C at the point in the cell cycle where the expression of the at temperature-sensitive cell division cycle(cdc)mutant, cdcX-. cdcX-grows normal normal cdc gene is required To determine where in the cell cycle expression of cdkX is required, you design experiments based on the following facts 1)The drug nocodazole arrests, but does not kill yeast in mitosis(M phase) 2)Cell density can be measured to determine if the yeast cells have completed a cell division. For your experiments You incubate cdkX- cells at 25 C with nocodazole until all the cells are synchronized. You then shift the cells to 36C and remove the nocodazole The cells divide once and then arrest You incubate cdkX-cells at 36 C until all arrest. You then add nocodazole and shift the cells to 25 The cells arrest without dividing. a)Given these experiments you can assume that the protein encoded by the cdkX gene is not required at what phase(s)of the cell?
3 Question 1 continued a) How do you explain the presence of sequences complementary to the oncogene in the DNA from healthy human and mouse samples? Why don't they have cancer? b) Why is the hybridizing band from sample 1 a different size than that from sample 5? c) For each cancer examined above, based on the autoradiogram, choose the most likely mechanism of transformation and explain your choice: 1) point mutation within the gene 2) chromosomal rearrangement involving the gene 3) gene amplification 4) oncogenic retroviral insertion. Question 2 You are studying the cell cycle in haploid yeast cells. You isolate a cell that is a temperature-sensitive cell division cycle (cdc) mutant, cdcX-. cdcX- grows normally at 25 °C, but arrests at 36 °C at the point in the cell cycle where the expression of the normal cdcX gene is required. To determine where in the cell cycle expression of cdkX is required, you design experiments based on the following facts: 1) The drug nocodazole arrests, but does not kill yeast in mitosis (M phase). 2) Cell density can be measured to determine if the yeast cells have completed a cell division. For your experiments: • You incubate cdkX- cells at 25 °C with nocodazole until all the cells are synchronized. You then shift the cells to 36 °C and remove the nocodazole. The cells divide once and then arrest. • You incubate cdkX- cells at 36 °C until all arrest. You then add nocodazole and shift the cells to 25 °. The cells arrest without dividing. a) Given these experiments you can assume that the protein encoded by the cdkX gene is not required at what phase(s) of the cell?
Another mutant cdkY-is a cold-sensitive mutant that arrests at 18C, but grows normally at 25C. cdkY-cells arrest between the s and G2 phases of the cell make cdkX-cdkY-double mutants and perform the following experiments. cycle. You You incubate cdkX-cdkY- double mutants at 36C until all the cells are synchronized and then shift the cells to 18 The cells arrest without dividing You incubate cdkX-cdkY-double mutants at 18C until all until all the cells are synchronized and then shift the cells to 360 The cells divide once and then arrest b)given these experiments you can assume that the protein encoded by the cdk X gene is not required at what phase(s)of the cell? c)In what stage(s)of the cell cycle do you expect the protein encoded by the cdkx gene to act? d)A cell in the process of DNA replication(cell A)is fused with a cell in early G1(cell B).How does the timing of the cell cycle for the cell B nucleus in the fused cell compare to that expected for the cell B nucleus if the cells remained independent? e)What soluble factor(s)are found in cell a but not in cell B(check all that apply)? pRB protein a camP dependent kinase TGFβ a G2 cyclin Human papilloma virus an S-phase cyclin a cyclin dependent kinase an oncogene a g1 cyclin
4 Another mutant cdkY- is a cold-sensitive mutant that arrests at 18 °C, but grows normally at 25 °C. cdkY- cells arrest between the S and G2 phases of the cell cycle. You make cdkX- cdkY- double mutants and perform the following experiments. • You incubate cdkX- cdkY- double mutants at 36 °C until all the cells are synchronized and then shift the cells to 18 °. • The cells arrest without dividing. • You incubate cdkX- cdkY- double mutants at 18 °C until all until all the cells are synchronized and then shift the cells to 36 °C. • The cells divide once and then arrest. b) Given these experiments you can assume that the protein encoded by the cdkX gene is not required at what phase(s) of the cell? c) In what stage(s) of the cell cycle do you expect the protein encoded by the cdkX gene to act? d) A cell in the process of DNA replication (cell A) is fused with a cell in early G1 (cell B). How does the timing of the cell cycle for the cell B nucleus in the fused cell compare to that expected for the cell B nucleus if the cells remained independent? e) What soluble factor(s) are found in cell A but not in cell B (check all that apply)? ___ pRB protein ___ a cAMP dependent kinase ___ TGFβ ___ a G2 cyclin ___ Human papilloma virus ___ an S-phase cyclin ___ a cyclin dependent kinase ___ an oncogene ____ a G1 cyclin
Question 3 Part i As a premier cancer biologist, you often plate cells in dishes, feeding them serum with growth factors and allowing them to grow for 2 weeks. Sometimes after incubation of strains you observe the following when looking at the side of a culture dish Strain a Strain b a)Which plate shows abnormal cells? Explain b)Predict the behavior of these cell lines if grown without added growth factors by drawing what the plates will look like after incubation without growth factors. Simply modify the existing figure below for your answer.(Note: one cell from each strain is initially deposited in each dish. Strain a Strain b Part ll A fellow researcher gives you two cancerous cell lines to examine and determine possible mutations. The results are shown below Cell Line Mutation WT none(wild type DNA 2 at the same region on both copies of chromosome 4 lutation in a gene on only one copy of chromosome 7 c) Based on this data above identify the type of cancer gene that is mutated in each of the cell lines Cell Line Cancer gene (oncogene or tumor suppressor gene) WT none 2
5 Question 3 Part I As a premier cancer biologist, you often plate cells in dishes, feeding them serum with growth factors and allowing them to grow for 2 weeks. Sometimes after incubation of strains you observe the following when looking at the side of a culture dish. Strain A Strain B a) Which plate shows abnormal cells? Explain. b) Predict the behavior of these cell lines if grown without added growth factors by drawing what the plates will look like after incubation without growth factors. Simply modify the existing figure below for your answer. (Note: one cell from each strain is initially deposited in each dish.) Strain A Strain B Part II A fellow researcher gives you two cancerous cell lines to examine and determine possible mutations. The results are shown below. Cell Line Mutation WT none (wild type DNA) 1 a deletion at the same region on both copies of chromosome 4 2 a point mutation in a gene on only one copy of chromosome 7 c) Based on this data above, identify the type of cancer gene that is mutated in each of the cell lines. Cell Line Cancer Gene (oncogene or tumor suppressor gene) WT none 1 2
Question 3 continued You learn that cell line l is a skin cancer cell line. The region you identified as deleted on chromosome 4 in these cells normally contains a gene called p16 d)What is the role of the p16 gene product in the normal cell based on the information above? You obtain another cell line(cell line 3)that has one wild-type copy of chromosome 4 and one mutant copy of chromosome 4(as described above in cell line 1) e)Will cell line 3 display a cancerous phenotype when grown in the presence of growth factors? Yes/No(Circle one Explain briefly f)Will cell line 3 display a cancerous phenotype when grown in the absence of growth factors? Yes/No(Circle one. Explain briefly Part Ill g)Cell line 2 is a breast cancer cell line that expresses a mutant version of a receptor protein called KIT. Choose from the following options to explain the role of KIT in normal cells Circle one Activation of kit causes cells to die Activation of Kit promotes progression through the cell cycle Activation of kit has no effect on the cell cycle Activation of kit causes cells to enter go h) Specifically how could a point mutation in the gene encoding the Kit receptor cause the abnormal behavior depicted in Part I
6 Question 3 continued You learn that cell line 1 is a skin cancer cell line. The region you identified as deleted on chromosome 4 in these cells normally contains a gene called p16. d) What is the role of the p16 gene product in the normal cell based on the information above? You obtain another cell line (cell line 3) that has one wild-type copy of chromosome 4 and one mutant copy of chromosome 4 (as described above in cell line 1). e) Will cell line 3 display a cancerous phenotype when grown in the presence of growth factors? Yes/No (Circle one.) Explain briefly. f) Will cell line 3 display a cancerous phenotype when grown in the absence of growth factors? Yes/No (Circle one.) Explain briefly. Part III g) Cell line 2 is a breast cancer cell line that expresses a mutant version of a receptor protein called KIT. Choose from the following options to explain the role of KIT in normal cells. Circle one. Activation of KIT causes cells to die Activation of KIT promotes progression through the cell cycle. Activation of KIT has no effect on the cell cycle. Activation of KIT causes cells to enter G0. h) Specifically how could a point mutation in the gene encoding the KIT receptor cause the abnormal behavior depicted in Part I
Question 4 you know in yeast that mitosis takes one hour you decide to further study the cell cycle in yeast cells using radioactive dTTP. Cells grown in radioactive dTTP incorporate this radioactive nucleotide into their DNA. you label a population of asynchronously growing yeast cells by adding radioactive dTTP to the medium for one minute. you then replace this medium with medium containing unlabelled dTTP and continue growing the cells. At one hour time points following the replacement you count the number of radioactively labeled cells in mitosis. your data is shown below 12000 0000 4000 234567891011121314151617181920212223 Time(in hours) after addition of unlabelled medium a)Cells are in which phase of the cell cycle when incorporating radioactive dTTP into their DNA?(Circle one. Go phase M phase Lunar phase b)Estimate the length of the G2 phase from the graph.(Circle one. O hrs 2-3 hrs 96-7 hrs 9-10 ~13-14hrs 20 hrs w22 hrs c)Estimate the length of the s phase from the graph.( Circle one. Can't o hrs N2-3 hrs -6-7 hrs 2 hrs d) Estimate the duration of the cell cycle. (Circle one Can't be O hrs w2-3 hrs *6-7 hrs ~9-10 11-12hrs ~13-14hrs c22 hrs e)Estimate the length of the G1 phase from the graph. ( Circle one. O hrs 2-3 hrs M6-7 hrs ~9-10 ~11-12hrs ~13-14hrs w22 hrs
7 Question 4 You know in yeast that mitosis takes one hour. You decide to further study the cell cycle in yeast cells using radioactive dTTP. Cells grown in radioactive dTTP incorporate this radioactive nucleotide into their DNA. You label a population of asynchronously growing yeast cells by adding radioactive dTTP to the medium for one minute. You then replace this medium with medium containing unlabelled dTTP and continue growing the cells. At one hour time points following the replacement you count the number of radioactively labeled cells in mitosis. Your data is shown below. 0 2000 4000 6000 8000 10000 12000 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 Time (in hours) after addition of unlabelled medium Number of labelled cells in mitosis a) Cells are in which phase of the cell cycle when incorporating radioactive dTTP into their DNA? (Circle one.) G0 phase G1 phase G2 phase M phase S phase Lunar phase b) Estimate the length of the G2 phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs c) Estimate the length of the S phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs d) Estimate the duration of the cell cycle. (Circle one) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs e) Estimate the length of the G1 phase from the graph. (Circle one.) Can’t be determined 0 hrs ~2-3 hrs ~6-7 hrs ~9-10 ~11-12 hrs ~13-14 hrs ~20 hrs ~22 hrs
Question 5 You are an immunologist who wants to make the big bucks. you decide to leave the world of science and get a job as a script-consultant on a new medical drama(Er-like) show. You test the writers with a few questions to see just how much they know a)Compare how macrophages and B cells recognize antigen b)Compare how macrophages and B cells present antigenic peptides(epitopes) They present epitopes exactly the same way on their MHC ll molecules on the surface c)Macrophages and B cells present antigens to cells (Fill in blanks.) d) Name 2 components of the innate or nonspecific immune system Below are short descriptions given to you by the writers of scenarios in the early Scenario #1 One of the characters on the show is diagnosed with leukemia, a cancer of the blood system. She is very sick until her boyfriend bravely agrees to donate his bone marrow e)You tell the writers that the bone marrow transplant from the boyfriend is unlikely to be successful. give the reason and the molecular basis for why Because of the different major histocompatability molecules the marrow will be rejected Scenario #2 The leading doctor gives birth to a baby boy. After some time, the child shows no acquired or specific immune response and is diagnosed with a rare disorder, Severe Combined Immune Deficiency(SCID), and as a result the boy must live in a germ-free environment. Several causes of SCid have been described and are listed below f) For each cause, indicate which of the following branches of immune system are affected ( Cellular=Cell-Mediated) Cause Cell-Mediated. humoral or both T cells fail to develop DNA recombination deficiency absence of mhc class i molecules Lack of mhc class ii molecules
8 Question 5 You are an immunologist who wants to make the big bucks. You decide to leave the world of science and get a job as a script-consultant on a new medical drama (ER-like) show. You test the writers with a few questions to see just how much they know. a) Compare how macrophages and B cells recognize antigen. b) Compare how macrophages and B cells present antigenic peptides (epitopes). They present epitopes exactly the same way on their MHC II molecules on the surface. c) Macrophages and B cells present antigens to ________ _____-cells. (Fill in blanks.) d) Name 2 components of the innate or nonspecific immune system. _______________________ _________________________ Below are short descriptions given to you by the writers of scenarios in the early episodes. Scenario #1 One of the characters on the show is diagnosed with leukemia, a cancer of the blood system. She is very sick until her boyfriend bravely agrees to donate his bone marrow. The bone marrow transplant is successful and our character lives! e) You tell the writers that the bone marrow transplant from the boyfriend is unlikely to be successful. Give the reason and the molecular basis for why. Because of the different major histocompatability molecules the marrow will be rejected. Scenario #2 The leading doctor gives birth to a baby boy. After some time, the child shows no acquired or specific immune response and is diagnosed with a rare disorder, Severe Combined Immune Deficiency (SCID), and as a result the boy must live in a germ-free environment. Several causes of SCID have been described and are listed below. f) For each cause, indicate which of the following branches of immune system are affected. (Cellular =Cell-Mediated) Cause Cell-Mediated, Humoral or Both T cells fail to develop DNA recombination deficiency Absence of MHC class I molecules Lack of MHC class II molecules
Scenario #3 Patients in the hospital are coming down with multiple infections. Lab results show that the sick are infected with a bacterium S aureus, that secretes"Protein a"which bind the constant region of antibodies g) What cell recognizes the constant region of secreted antibodies? h) Why might the effect of Protein A allow multiple(non S aureus)infections? i)What branch of the immune system does S aureus evade using Protein A? Humoral Cellular or Cell-Mediated Both enario #4 Many patients are coming into the emergency room with a disease caused by an unknown pathogen! a doctor studies this pathogen in order to create a vaccine against j) He discovers that the infectious agent is an intracellular bacterium and its cell surface is coated with human-like proteins. Considering the mechanism of the pathogen, the doctor decides to generate a live-attenuated vaccine instead of a heat-killed vaccine i)What are the two advantages of using a live-attenuated vaccine vs a heat killed accine in this case? ii What is a disadvantage of using a live-attenuated vaccine? Question 6 a) Shown below is a schematic of the production of a heavy chain polypeptide for an the bottom is shown the heavy chain polypeptide ent found antibody. At the top is the chromosomal arrangement found in an immature B cell, at i)Label the process indicated by each arrow. Choose the one best option for each protein processing RNA ligation transcription A splicing translation DNA rearrangement transduction DNA ligation ii)Indicate on the diagram below where you would expect to find each of the following components, Promoter Transcription terminator start codon stop codon di) Indicate on the diagram below the variable and the constant region of the heavy chain polypeptide
9 Scenario #3 Patients in the hospital are coming down with multiple infections. Lab results show that the sick are infected with a bacterium, S. aureus, that secretes “Protein A” which binds the constant region of antibodies. g) What cell recognizes the constant region of secreted antibodies? ____________ h) Why might the effect of Protein A allow multiple (non S. aureus) infections? i) What branch of the immune system does S. aureus evade using Protein A? Humoral Cellular or Cell-Mediated Both Scenario #4 Many patients are coming into the emergency room with a disease caused by an unknown pathogen! A doctor studies this pathogen in order to create a vaccine against it. j) He discovers that the infectious agent is an intracellular bacterium and its cell surface is coated with human-like proteins. Considering the mechanism of the pathogen, the doctor decides to generate a live-attenuated vaccine instead of a heat-killed vaccine. i) What are the two advantages of using a live-attenuated vaccine vs. a heat killed vaccine in this case? ii) What is a disadvantage of using a live-attenuated vaccine? Question 6 a) Shown below is a schematic of the production of a heavy chain polypeptide for an antibody. At the top is the chromosomal arrangement found in an immature B cell, at the bottom is shown the heavy chain polypeptide. i) Label the process indicated by each arrow. Choose the one best option for each from: protein processing RNA ligation transcription RNA splicing translation DNA rearrangement transduction DNA ligation ii) Indicate on the diagram below where you would expect to find each of the following components; Promoter Transcription terminator start codon stop codon iii) Indicate on the diagram below the variable and the constant region of the heavy chain polypeptide
=intron regions b)Indicate whether each of the following statements is true or false. If false correct the statement or provide a brief explanation for why it is false i)Memory cells are the basis for a strong secondary humoral immune response ii)T cells produce antibodies that bind antigen ii)Macrophages present antigenic peptides on MHC II proteins to B cells iv)Clonal expansion means that any B cells present at the time of exposure to an antigen will be stimulated to proliferate. v) The two antigen binding sites formed by the variable regions in a single antibody molecule bind to two different antigenic determinants c)When a rabbit protein is injected into rabbits, no antibodies against this protein are generated. If, however the same rabbit protein is injected into guinea pigs, the guinea pigs generate antibodies against the rabbit protein. Briefly (in one or two sentences) explain this observation
10 V segments D segments J segments constant segment = intron regions b) Indicate whether each of the following statements is true or false. If false, correct the statement or provide a brief explanation for why it is false. i) Memory cells are the basis for a strong secondary humoral immune response. ii) T cells produce antibodies that bind antigen. iii) Macrophages present antigenic peptides on MHC II proteins to B cells. iv) Clonal expansion means that any B cells present at the time of exposure to an antigen will be stimulated to proliferate. v) The two antigen binding sites formed by the variable regions in a single antibody molecule bind to two different antigenic determinants. c) When a rabbit protein is injected into rabbits, no antibodies against this protein are generated. If, however, the same rabbit protein is injected into guinea pigs, the guinea pigs generate antibodies against the rabbit protein. Briefly (in one or two sentences) explain this observation