Lecture 3: Degradable Materials with Biological Recognition Last time Theory of hydrolytic polymer erosion Enzymatic degradation of polymers Designing Biodegradable Macromolecules Today Biological recognition in vivo Engineering biological recognition of biomaterials: cell adhesion/migration Readin S.E. Sakiyama-Elbert and J. A Hubbell, ' Functional Biomaterials: Design of Novel biomaterials, Annu. Rev. Mater. Sci. 31, 183-201(2001) J.C. Schense et aL., 'Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension, Nat. Biotech. 18, 415-419 (2000) Supplementary Reading: The Extracellular Matrix, pp. 1124-1150, Molecular Biology of the Cel, Lodish et al Biological Recognition in vivo Interactions of cells with their environment at the molecular level ECM extracellular matrix Motivation Cell interactions with simple synthetic materials are governed by nonspecific interactions o e.g. surface energies; hydrophobic interactions, charge-charge interactions DRAW OXIDE SURFACE. POL YMER SURFACE but this is not how cells interact with ecm Cells use receptor-receptor/receptor-ligand interactions to guide their functions, including o Adhesion, migration o Differentiation Secretion of molecules Binding of molecules Functions of ECM · Mechanical Support cues for cell survival/function o anchorage-dependent cell growth o differentiation cues organization of tissue o control of tissue morphology, localization of cell types Lecture 3- Biological Recognition 1of15Lecture 3: Degradable Materials with Biological Recognition Last time: Theory of hydrolytic polymer erosion Enzymatic degradation of polymers Designing Biodegradable Macromolecules Today: Biological recognition in vivo Engineering biological recognition of biomaterials: cell adhesion/migration Reading: S.E. Sakiyama-Elbert and J.A. Hubbell, ‘Functional Biomaterials: Design of Novel biomaterials,’ Annu. Rev. Mater. Sci. 31, 183-201 (2001) J.C. Schense et al., ‘Enzymatic incorporation of bioactive peptides into fibrin matrices enhances neurite extension,’ Nat. Biotech. 18, 415-419 (2000) Supplementary Reading: ‘The Extracellular Matrix,’ pp. 1124-1150, Molecular Biology of the Cell, Lodish et al. Biological Recognition in vivo Interactions of cells with their environment at the molecular level ECM = extracellular matrix Motivation: • Cell interactions with simple synthetic materials are governed by nonspecific interactions: o e.g. surface energies; hydrophobic interactions, charge-charge interactions o DRAW OXIDE SURFACE, POLYMER SURFACE • …but this is not how cells interact with ECM • Cells use receptor-receptor/receptor-ligand interactions to guide their functions, including: o Adhesion, migration o Growth o Differentiation Secretion of molecules Binding of molecules Specialized functions Functions of ECM: • Mechanical Support • cues for cell survival/function o anchorage-dependent cell growth o differentiation cues • organization of tissue o control of tissue morphology, localization of cell types Lecture 3 – Biological Recognition 1 of 15