Structure of native ecm scaffold Prototy pical soft tissue ECM(varies from tissue to tissue) o collagen o other fibers SLIDE) reticular network collagen gel TEM of collagen fibril generalized matrix assembly o fibrils assemble into fibers fibers may be organized or isotropic, and form tight(-10 nm separation between fibers)or open (20-30 um between fibers)meshes o adhesion proteins decorate'fibers o other signals(cytokines, etc )may also be sequestered on fiber surfaces Sequestered Cytokines/ Chemokines/ Collagen fiber hydrogel Adhesion motifs P Friedl et al. Eur J Immuno. 28, 2331(1998) collagen triple helices Lecture 3- Biological Recognition 2of15Structure of native ECM scaffold Prototypical soft tissue ECM (varies from tissue to tissue): • structural fibers o collagen o other fibers? (SLIDE) collagen gel reticular network of lymph node TEM of collagen fibril • generalized matrix assembly: o fibrils assemble into fibers ƒ fibers may be organized or isotropic, and form tight (~10 nm separation between fibers) or open (20-30 µm between fibers) meshes o adhesion proteins ‘decorate’ fibers o other signals (cytokines, etc.) may also be sequestered on fiber surfaces Collagen fiber hydrogel P. Friedl et al.: Eur. J. Immunol. 28, 2331 (1998). Collagen triple helices Collagen fiber Adhesion motifs Sequestered Cytokines/ Chemokines/ Etc. Lecture 3 – Biological Recognition 2 of 15