Specimen Gram stain> gram-negative rods and or white blood cells Diagnosis Clear colonies on mac conkey agar “脓液、创面渗出液、痰、尿和血· Confirmatory tests: production of the 等标本,直接接种于血琼脂平板, blue-green pigment pyocyanin on cetrimide 根据菌落大小,绿色色素及生化反 应等鉴定。 garand growth at42℃C 《医学微生物学》P170·TS| slant: distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens When P aeruginosa is isolated from a normally sterile site(blood, bone, deep collections) it is generally considered dangerous, and almost always requires treatment. However, P aeruginosa is frequently isolated from nonsterile sites (mouth swabs, sputum, etc. ),and, under these circumstances, it may represent colonization and not infectionDiagnosis • Specimen + Gram stain → gram-negative rods and/or white blood cells • Clear colonies on MacConkey agar • Confirmatory tests： production of the blue-green pigment pyocyanin on cetrimide agarand growth at 42℃ • TSI slant ：distinguish nonfermenting Pseudomonas species from enteric pathogens in faecal specimens. • When P. aeruginosa is isolated from a normally sterile site (blood, bone, deep collections), it is generally considered dangerous, and almost always requires treatment. However, P. aeruginosa is frequently isolated from nonsterile sites (mouth swabs, sputum, etc.), and, under these circumstances, it may represent colonization and not infection. “脓液、创面渗出液、痰、尿和血 等标本，直接接种于血琼脂平板， 根据菌落大小，绿色色素及生化反 应等鉴定。” ——《医学微生物学》P170