蛋白质浴液检测红外- 最大的“敌人” 与安被打 水的红外光谱 0.8 0.6 如何解决？ 0.4 0.2 FTIR spectroscopy Aggregates were collected at the end of aggregation reaction by centrifugation at 14,000 r.p.m.for 30 min.The pellet is washed once(PBS plus centrifugation),resuspended in~50ul of 1x PBS,and the suspension placed between two polished CaF2 windows using a BioCell module(BioTools,Inc.)on an ABB Prota-2x MB 3000 FTIR instrument.Spectra were collected at 4 cm-1 resolution (average of 400 scans)and corrected for buffer absorption until a flat baseline was obtained in the 1,700-1,800 cm region.Second derivative spectra for the amide-I region were calculated using PROTA software.蛋白质溶液检测红外 ------ 最大的“敌人” 如何解决？ 严禁复制