Prediction of gene expression changes In silico gene expression change predictions were compared to experimentally measured expression profiles as well as experimentally determined protein DNA interactions(ChIP-chip)and predicted TF-binding motifs to assess the completeness of the MH805/775 network Gene expression data for eight transcription factor knockout strains(rgt1, rox1, gat1, hap1, adr1, gal4, gIn3 cat8 and two overexpression(HAP4, GCN4) strains from previously published reports were used Each of genes was classified as significantly up- regulated, significantly down-regulated, or unchanged in each of the 10 experimental data setsPrediction of gene expression changes • In silico gene expression change predictions were compared to experimentally measured expression profiles as well as experimentally determined protein– DNA interactions (ChIP-chip) and predicted TF-binding motifs to assess the completeness of the iMH805/775 network. • Gene expression data for eight transcription factor knockout strains (rgt1, rox1, gat1, hap1, adr1, gal4, gln3, cat8) and two overexpression (HAP4, GCN4) strains from previously published reports were used • Each of genes was classified as significantly up￾regulated, significantly down-regulated, or unchanged in each of the 10 experimental data sets