NMR data 1:NOE For short mixing times NOE cross peak intensity is proportional to 1/r6 of two protons. 。NOE~1/r6ftc) For well structured areas of a macromolecule f(t)can be considered to be constant.(in practice this is assumed to be true for all parts of the molecule) -Calibration of cross peaks by using a proton pair of known local geometry (distance) -Because of multiple simplifying assumptions of the relationship between NOE and distance it is usually used only qualitatively(class NOEs in three bins:strong,medium and weak) 14NMR data 1: NOE • For short mixing times NOE cross peak intensity is proportional to 1/r6 of two protons. • NOE ~ 1/r6 f(tc ) – For well structured areas of a macromolecule f(tc ) can be considered to be constant. (in practice this is assumed to be true for all parts of the molecule) – Calibration of cross peaks by using a proton pair of known local geometry (distance) – Because of multiple simplifying assumptions of the relationship between NOE and distance it is usually used only qualitatively (class NOEs in three bins: strong, medium and weak) 14