Transition state,X* Note:AG*does not enter △Gt(uncatalyzed) into the final△G △G(catalyzed) calculation for the reaction, Substrate K6Jeua because the energy input △G for the required to reach the reaction transition state is returned when the transition state Product forms the product.△G*不 Reaction progress→ 参与到反应自由能，因为 形成时所需自由能和裂解 时释放自由能相抵消 Enzymes function:to lower the activation energy,or, in other words,enzymes facilitate the formation of the transition state.Enzymes function： to lower the activation energy, or, in other words, enzymes facilitate the formation of the transition state. Note： ΔG* does not enter into the final ΔG calculation for the reaction, because the energy input required to reach the transition state is returned when the transition state forms the product. ΔG*不 参与到反应自由能，因为 形成时所需自由能和裂解 时释放自由能相抵消