Culture Korthof medium with 10%/ rabbit serum The optimal incubating temperature is about 28C L, interrogans grows slowly Sensitive to heating and various chemical agents Can survive several months in wet soil and water OL interrogans has two chromosomes(one is large and the other is small).17 Culture • Korthof medium with 10% rabbit serum • The optimal incubating temperature is about 28℃ • L. interrogans grows slowly • Sensitive to heating and various chemical agents • Can survive several months in wet soil and water ◇L. interrogans has two chromosomes (one is large and the other is small)