Key points: DNA-mediated transformation. Difficult points: gene transfer by viral transduction Chapter ll Genetic manipulation of animals To have a good grasp of genetic manipulation of mammals. To be familiar with DNA transfer to other vertebrates dna transfer to invertebrates Key points: genetic manipulation of mammals Difficult points: genetic manipulat Chapter 12 Gene transfer to plants To have a good grasp of Agrobacterium-mediated transformation. To be familiar with chloroplast transformation, plant viruses as vectors. To know the content of direct DNA transfer to Key points: Agrobacterium-mediated transformation Difficult points: chloroplast transformation Chapter 13 Advances in transgenic technology To have a good grasp of further transgenic strategies for gene inhibition, inducible expression systems. To be familiar with applications of site-specific recombination. To know the content of Key points: further transgenic strategies for gene inhibition, applications of site-specific recombination Difficult points: applications of site-specific recombination Chapter 14 Application of recombinant DNA tecgnology To have a good grasp of nucleic acid sequences as diagnostic tools, protein engineering and metabolic engineering. To be familiar with plant breeding in the twenty-first century and new drugs and new therapies for genetic diseases Key points: nucleic acid sequences as diagnostic tools, protein engineering and metabolic 五、教学时数分配表 Chapter Content 参考时数 Gene manipulation: an all-embracing technique Basic technic Cutting and joining dna molecules Basic biology of plasmid and phage vectors Cosmids, phasmids and other advanced vectors 7 Sequencing and mutagenesis Cloning in bacteria other than Escherichia col 9 Cloning in Saccharomyces cerevisiae and other fun http:/spxy.zjgsu.edu.cnhttp://spxy.zjgsu.edu.cn Key points：DNA-mediated transformation. Difficult points：gene transfer by viral transduction. Chapter 11 Genetic manipulation of animals To have a good grasp of genetic manipulation of mammals. To be familiar with DNA transfer to other vertebrates, DNA transfer to invertebrates. Key points：genetic manipulation of mammals. Difficult points：genetic manipulation of mammals. Chapter 12 Gene transfer to plants To have a good grasp of Agrobacterium-mediated transformation. To be familiar with chloroplast transformation, plant viruses as vectors. To know the content of direct DNA transfer to plant. Key points：Agrobacterium-mediated transformation. Difficult points：chloroplast transformation. Chapter 13 Advances in transgenic technology To have a good grasp of further transgenic strategies for gene inhibition, inducible expression systems. To be familiar with applications of site-specific recombination. To know the content of transgenic technology for functional genomics. Key points：further transgenic strategies for gene inhibition, applications of site-specific recombination. Difficult points：applications of site-specific recombination. Chapter 14 Application of recombinant DNA tecgnology To have a good grasp of nucleic acid sequences as diagnostic tools, protein engineering and metabolic engineering. To be familiar with plant breeding in the twenty-first century and new drugs and new therapies for genetic diseases. Key points：nucleic acid sequences as diagnostic tools, protein engineering and metabolic engineering. 五、教学时数分配表 Chapter Content 参考时数 1 Gene manipulation: an all-embracing technique 2 2 Basic techniques 2 3 Cutting and joining DNA molecules 2 4 Basic biology of plasmid and phage vectors 2 5 Cosmids, phasmids and other advanced vectors 2 6 Cloning strategies 2 7 Sequencing and mutagenesis 2 8 Cloning in bacteria other than Escherichia coli 4 9 Cloning in Saccharomyces cerevisiae and other fungi 4