Chapter 4 Basic biology of plasmid and phage vectors To have a good grasp of the plasmid biology and simple plasmid vectors, bacteriophage n To be familiar with the content of the purification of plasmid DNA. To know the content of DNA cloning with single-stranded dna vecters Key points: the plasmid biology and simple plasmid vectors, bacteriophage h Difficult points: bacteriophage Chapter 5 Cosmids, phasmids and other advanced vectors To have a good grasp of vectors for cloning large fragments of DNA. To be familiar with the content of specialist-purpose vectors. To know the content of putting it all together: vectors with bination of features Key points: vectors for cloning large fragments of DNA Difficult points: vectors with combination of features Chapter 6 Cloning strategies To have a good grasp of cloning genomic DNA. To be familiar with the cDNA clonin screening strategies. To know the content of difference cloning Key points: cloning genomic DNA Difficult points: screening strategies Chapter 7 Sequencing and mutagenesis To have a good grasp of basic DNA sequencing, whole-genome sequencing. To be familiar with analyzing sequencing data. To know the content of changing genes: site-directed mutagensis Key points: basic DNA sequencing, whole-genome sequencing Difficult points: changing genes: site-directed mutagens Chapter 8 Cloning in bacteria other than Escherichia coli To have a good grasp of introducing DNA into bacterial cells. To be familiar with cloning in Gram-negative bacteria other than E. coli, cloning in Gram-positive bacteria. To know the content of cloning in streptomycetes Key points: introducing DNA into bacterial cell Difficult points: cloning in streptomycetes Chapter 9 Cloning in Saccharomyces cerevisiae and other fungi To have a good grasp of introducing DNA into fungi, expression of cloned genes. To be amiliar with yeast surface display. To know the content of identifying genes encoding particular cellular activities, determining functions associated with particular genes Key points: introducing DNA into fungi, expression of cloned genes Difficult points: yeast surface display Chapter 10 Gene transfer to animal cells To have a good grasp of DNA-mediated transformation, gene transfer by viral transduction To be familiar with expression systems for animal cell. http:/spxy.zjgsu.edu.cnhttp://spxy.zjgsu.edu.cn Chapter 4 Basic biology of plasmid and phage vectors To have a good grasp of the plasmid biology and simple plasmid vectors, bacteriophage λ. To be familiar with the content of the purification of plasmid DNA. To know the content of DNA cloning with single-stranded DNA vecters. Key points：the plasmid biology and simple plasmid vectors, bacteriophage λ. Difficult points：bacteriophage λ. Chapter 5 Cosmids, phasmids and other advanced vectors To have a good grasp of vectors for cloning large fragments of DNA. To be familiar with the content of specialist-purpose vectors. To know the content of putting it all together: vectors with combination of features. Key points：vectors for cloning large fragments of DNA. Difficult points：vectors with combination of features. Chapter 6 Cloning strategies To have a good grasp of cloning genomic DNA. To be familiar with the cDNA cloning, screening strategies. To know the content of difference cloning. Key points：cloning genomic DNA. Difficult points：screening strategies. Chapter 7 Sequencing and mutagenesis To have a good grasp of basic DNA sequencing, whole-genome sequencing. To be familiar with analyzing sequencing data. To know the content of changing genes:site-directed mutagensis. Key points：basic DNA sequencing, whole-genome sequencing. Difficult points：changing genes:site-directed mutagensis. Chapter 8 Cloning in bacteria other than Escherichia coli To have a good grasp of introducing DNA into bacterial cells. To be familiar with cloning in Gram-negative bacteria other than E. coli, cloning in Gram-positive bacteria. To know the content of cloning in streptomycetes. Key points：introducing DNA into bacterial cells. Difficult points：cloning in streptomycetes. Chapter 9 Cloning in Saccharomyces cerevisiae and other fungi To have a good grasp of introducing DNA into fungi, expression of cloned genes. To be familiar with yeast surface display. To know the content of identifying genes encoding particular cellular activities, determining functions associated with particular genes. Key points：introducing DNA into fungi, expression of cloned genes. Difficult points：yeast surface display. Chapter 10 Gene transfer to animal cells To have a good grasp of DNA-mediated transformation, gene transfer by viral transduction. To be familiar with expression systems for animal cell