《基因工程原理和技术(英)》教学大纲 (Genetic Engineering 基本信息 课程代码:1040022 学分:2 总课时:32 课程性质:专业选修课 适用专业:生物工程 先修课程:生物化学,微生物学,分子生物学 、本课程教学目的和任务 基因工程原理与技术是为生物工程专业开设的一门专业选修课。通过课程学习,使学生 更进一步了解生物技术领域中的前沿技术,并扎实专业理论知识。 三、教学方法与手段 多媒体教学。 四、教学内容及要求 Chapter 1 Gene manipulation: an all-embracing technique To have a good grasp of the concepts of gene manipulation and content. To be familiar with the content of sequence analysis, in vivo biochemistry, the new medicine, biotechnology, the central role of E. coli. To have an idea of the historical development of genetic engineering Key points: The concepts of gene manipulation and content Chapter 2 Basic techniques To have a good grasp of the techniques of agarose gel electrophoresis, nucleic acid blotting ansformation of E coli and PCR. To be familiar with the content of real-time quantitative PCR To know the content of transformation of other organisms Key points: transformation of E coli and PCr Difficult points: real-time quantitative PCR. Chapter 3 Cutting and joining DNA molecules To have a good grasp of the joining DNA molecules. To be familiar with the content host-controlled restriction and modification. To know the content of the Dam and Dcm methylas of E. coli, the importance of eliminating restriction systems in E. coli strains used as hosts for recombinant molecules Key points: the joining DNA molecules Difficult points host-controlled restriction and modification, and the Dam and dcm methylases of E coli http:/spxy.zjgsu.edu.cnhttp://spxy.zjgsu.edu.cn 《基因工程原理和技术（英）》教学大纲 (Genetic Engineering) 一、基本信息 课程代码：1040022 学 分：2 总 课 时：32 课程性质：专业选修课 适用专业：生物工程 先修课程：生物化学，微生物学，分子生物学 二、本课程教学目的和任务 基因工程原理与技术是为生物工程专业开设的一门专业选修课。通过课程学习，使学生 更进一步了解生物技术领域中的前沿技术，并扎实专业理论知识。 三、教学方法与手段 多媒体教学。 四、教学内容及要求 Chapter 1 Gene manipulation: an all-embracing technique To have a good grasp of the concepts of gene manipulation and content. To be familiar with the content of sequence analysis, in vivo biochemistry, the new medicine, biotechnology, the central role of E. coli. To have an idea of the historical development of genetic engineering. Key points：The concepts of gene manipulation and content. Chapter 2 Basic techniques To have a good grasp of the techniques of agarose gel electrophoresis, nucleic acid blotting, transformation of E. coli and PCR. To be familiar with the content of real-time quantitative PCR. To know the content of transformation of other organisms. Key points：transformation of E. coli and PCR. Difficult points：real-time quantitative PCR. Chapter 3 Cutting and joining DNA molecules To have a good grasp of the joining DNA molecules. To be familiar with the content of host-controlled restriction and modification. To know the content of the Dam and Dcm methylases of E. coli , the importance of eliminating restriction systems in E. coli strains used as hosts for recombinant molecules. Key points：the joining DNA molecules. Difficult points：host-controlled restriction and modification, and the Dam and Dcm methylases of E. coli