A plot of l/Versus 1/[S], called a Lineweaver- Burk plot, yields a straight line with an intercept of 1/Vmax and a slope of KM/Vmax(Figure 8-16) Alternatively, KM and Vmax can be obtained by fitting the data to equa- tion 28 using a computer program Part Il A plot of 1/Versus 1/[S, called a Lineweaver- Burk plot, yields a straight PROTEINS line with an intercept of l/Vmax and a slope of KM/Vmax( Figure 8-16) Alternatively, KM and Vmax can be obtained by fitting the data to equa tion 28 using a computer program Figure 8-16 double-reciprocal plot of enzyme kinetics: 1/v is plotted as a function of 1/[S]. The slope is KM/Vmax Intercept =-1/KM Intercept =1/vna intercept on the vertical axis is 1/Vmax, and the intercept on the horizontal axis is -1/KM