Vmax and Km cAn BE DETERMINED BY VARYING THE SUBSTRATE CONCENTRATION The Michaelis constant, KM, and the maximal rate, Vmax, can be readily derived from rates of catalysis measured at different substrate concentra- tions if an enzyme operates according to the simple scheme given in equation 14. It is convenient to transform the Michaclis-Menten equation into one that gives a straight-line plot. This can be done by taking the reciprocal of both sides of equation 28 to give KM 99 max max S The Lineweaver-Burk plot [S] V= V max [S]+ KM (28)The Lineweaver-Burk plot