Affinity Purification What do you mean by an " interaction? Most proteins interact with several other proteins(estimate 2-10) Many proteins in the cell are found in complexes. for some purposes knowing the identities of the members of the clusters is as useful, or more useful, than knowing the directly interacting partners. Affinity purification is a method for characterizing the clusters directly, rather than one interaction at a timeAffinity Purification What do you mean by an “interaction”? Most proteins interact with several other proteins (estimate 2-10). Many proteins in the cell are found in complexes. For some purposes, knowing the identities of the members of the clusters is as useful, or more useful, than knowing the directly interacting partners. Affinity purification is a method for characterizing the clusters directly, rather than one interaction at a time