Affinity Purification/Mass spectrometry DNA encodes bait tag a C BAIT bait expressed in cell forms part of a se cell, fish for complex complex with affinity column that binds a separate affinity tac a, b, d, e bait C BAIT by sds page gel extract bands identities digest with trypsin proteins PEPTIDES in the mass spec t complex database searchAffinity Purification/Mass spectrometry affinity tag BAIT a b c d e BAIT a b c d e DNA encodes bait + tag bait expressed in cell forms part of a complex complex with affinity the tag separate a,b,c,d,e,BAIT extract bands, digest with trypsin PEPTIDES mass spec + identities of proteins in the complex lyse cell, fish for column that binds by SDS PAGE gel database search