BEH. 462/3.962J Molecular Principles of Biomaterials Spring 2003 Dual growth factor delivery from degradable scaffolds for de novo blood vessel synthesis Nacl particles · Fabrication process o PDGF encapsulated in PLGA microspheres by double emulsion approach o Microspheres(5-50 um) mixed with PLGA particles(150-250 um), Nacl particles(250-500 um), and lyophilized VEGF particles(5-50 um) in mold and compression molded to form a solid disk Disk equilibrated with COz at 800 psi 48hrs o Pressure rapidly dropped to ambient (14 psi) o Salt leached by soaking in distilled water 48 hrs 2. Rapidly depressurize Soak with water to leach out Nacl Lecture 5- Programmed Drug Delivery 9 of 12BEH.462/3.962J Molecular Principles of Biomaterials Spring 2003 Lecture 5 – Programmed Drug Delivery 9 of 12 • Dual growth factor delivery from degradable scaffolds for de novo blood vessel synthesis9 : Compression mold PLGA particles Lyophilized VEGF PDGF-containing microspheres NaCl particles • Fabrication process:10 o PDGF encapsulated in PLGA microspheres by double emulsion approach o Microspheres (5-50 µm) mixed with PLGA particles (150-250 µm), NaCl particles (250-500 µm), and lyophilized VEGF particles (5-50 µm) in mold and compression molded to form a solid disk o Disk equilibrated with CO2 at 800 psi 48hrs o Pressure rapidly dropped to ambient (14 psi) o Salt leached by soaking in distilled water 48 hrs 1. Fill with high-pressure CO2 2. Rapidly depressurize Soak with water to leach out NaCl